Abstract: The genome of FootandMouth disease virus (FMDV) O/LZ isolate was cloned by RT-PCR, and sequenced. The result showed that the genome of O/LZ isolate is 8 014nt in length, including partical 5′UTR and complete polyprotein coding region, and their nucleotide sequence are 1 042 nt and 6 969nt respectively. Comparison between O/LZ and other reference isolates indicates that O/LZ strain have a close evolutionary relationship with O/HNK/2002, O/ES/2001,O/CHP/TW/97,etc,O/LZ should be classified to Cathay topotype . Furthermore, comparison of deduced amino acid residues at the antigenic sites with PanAsia strain of FMDV serotype O ME-SA topotype shows there are obviously topotypical amino acid difference at Site 1 and Site 3, thus, suggesting the less antigenic relationships between these two topotype isolates.