畜牧兽医学报 ›› 2006, Vol. 37 ›› Issue (5): 518-522.doi:

• 研究简报 • 上一篇    

温氏附红细胞体16S rRNA基因的序列测定和分析

付 媛;李树清;杜 凯;刘 琴;周艳琴;刘恩勇;姚宝安;赵俊龙
  

  1. 1. 华中农业大学农业微生物学国家重点实验室，武汉 430070;2. 华中农业大学动物医学院，武汉 430070; 3. 上海进出口检验检疫局，上海 200135;4. 湖北省兽疫防治站，武汉 430064; 5. 浙江省农科院畜牧兽医研究所，杭州 310021
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-05-25 发布日期:2006-05-25

Sequence and Molecular Characterization of 16S rRNA Gene of Eperythrozoon wenyonii-Hubei Strain

FU Yuan;LI Shu-qing;DU Kai;LIU Qin;ZHOU Yan-qin;LIU En-yong;YAO Bao-an;ZHAO Jun-long   

  1. 1. National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070,China; 2. College of Veterinary Medicine, Huazhong Agricultural University,Wuhan 430070,China;3. Shanghai Export and Import Inspection and Quarantine Bureau, Shanghai 200135,China;4. Hubei Veterinary Prevention and Control Station, Wuhan 430064,China;5. Research Institute of Animal Science and Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-05-25 Published:2006-05-25

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摘要： 从自然感染附红细胞体的湖北黄牛无菌采集血液，分离附红细胞体并提取病原基因组，用血营养菌的16S rRNA 基因的通用引物进行PCR扩增，得到长约1.5 kb的扩增片段，将其克隆到pMD18-T载体后进行测序和分析。结果表明该片段全长为1 471 bp（GenBank收录号为AY946266），同源性分析表明该序列与Neimark公布的温氏附红细胞体(AF016546)的16S rRNA 基因序列同源性达到98.7％，证实该病原为温氏附红细胞体，从分子生物学水平证实了温氏附红细胞体在湖北省的存在。将该序列与5种支原体、14种血营养菌及边缘无浆体等的相应序列进行比较，建立系统发育树，结果表明温氏附红细胞体同边缘无浆体的关系较远，而与肺炎支原体组的亲缘关系较近。

Abstract: The complete 16S rRNA gene of E.wenyonii-Hubei strain was determined by PCR. It was sequenced and blasted. The results showed that the sequence was 98.7% identical to that of Neimark published sequence（AF016546）. The 1 471bp complete sequence was compared with the sequence of 5 mycoplasmas,14 haemotrophic bacteria and Anaplasma marginale available in GenBank. The data were analyzed and a phylogenetic tree was established. The results indicated that the hereditary distance of E.wenyonii was close to that of pneumoniae group，and the hereditary distance was far from Anaplasma marginale.

ISSN 0366-6964
CN 11-1985/S
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