Abstract: The aim of this study was to prepare the antiserums against chicken livertype fatty acid binding protein (L-FABP) and analyze expression characteristics of L-FABP. Specific primers were designed to amplify the gene of chicken L-FABP by RT-PCR. The L-FABP gene was then inserted into pGEX4T-1 vector and expressed in Escherichia coli BL21 (DE3). The protein was purified by Glutathione Sepharose 4B affinity chromatography and the antiserums against L-FABP was produced by immunizing rabbits. The tissue expression characteristics of L-FABP gene were determined by Western blot analysis. The results showed that a 40 ku (14 ku L-FABP + 26 ku GST) fusion protein was induced; furthermore, high titer antiserums against L-FABP was obtained. The tissue expression analysis showed that L-FABP was highly expressed in liver and small intestine but no hybridization signal was detected in heart, fat, muscle, muscle stomach, spleen, lung and kidney.