雌激素对去卵巢大鼠小脑ER、ChAT、NGF表达的影响

畜牧兽医学报 ›› 2007, Vol. 38 ›› Issue (9): 959-965.doi:

雌激素对去卵巢大鼠小脑ER、ChAT、NGF表达的影响

罗启慧;陈正礼;范光丽

1四川农业大学动物科技学院，雅安 625014；2西北农林科技大学动物科技学院，杨凌 712100

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-09-25 发布日期:2007-09-25

Effects of Estrogen on Expression of ER, NGF and ChAT in Cerebellum of Ovariectomized Female Sprague-Dawley Rat

LUO Qi-hui;CHEN Zheng-li;FAN Guang-li

1 College of Animal Science and Technology, Sichuan Agricultural University, Ya’an 625014,China; 2 College of Animal Science and Technology, Northwest A & F University, Yangling 712100,China

Received:1900-01-01 Revised:1900-01-01 Online:2007-09-25 Published:2007-09-25

摘要/Abstract

摘要： 本研究旨在探讨雌激素对大鼠小脑内雌激素受体（ER）、神经生长因子（NGF）和胆碱乙酰转移酶（ChAT）表达的影响。建立去卵巢SD大鼠模型，以小脑为研究对象，运用免疫组织化学SP法，通过补充17β-雌二醇对ER、NGF和ChAT在小脑中的表达和分布变化进行研究。结果表明： ER、NGF和ChAT免疫阳性反应物在小脑皮质主要分布于蒲肯野氏细胞，而在小脑核中主要定位于小脑顶核、间位核和齿状核；ER阳性产物主要定位于细胞质和突起中，也存在于胞膜和胞核中。去卵巢大鼠小脑中3种阳性物质的表达强度及阳性细胞数量总体呈显著降低趋势，在补充17β－雌二醇后，3种阳性物质的表达强度和阳性细胞数目显著回升。本试验证实雌激素可促进小脑中NGF和ChAT的表达；另外ER、NGF和ChAT表达变化的相似性提示三者在雌激素对小脑的作用中是相互调节和影响的，同时表明雌激素可能既通过基因组机制，也通过非基因组机制途径在小脑发挥作用。

Abstract: The effects of estrogen on the expression of ER, NGF and ChAT in rats cerebellum were studied in this paper.The model of ovariectomized female rat was established to study the expression and distribution of estrogen receptor (ER), nerve growth factor (NGF) and choline acetyltransferase (ChAT) in the cerebellum after 17β-estradiol treatment by using technique of immunohistochemical ultrasensitive SP in Sprague.Dawley ratThe immunoreactive products were distributed in Purkinje′s cell, dentate nucleus, interstitial nucleus and fastigial nucleus of cerebellum, and the ER positive products mainly located in plasma, cytoplasmic membrane and neurite, also existed in nucleus.The general tendency of the expression of ER, NGF and ChAT positive products in cerebellar cortex and nuclei of ovariectomized rat is significantly decreased, while the intensity and quantity of the immunoreactive products ascended predominantly after 17β-estradiol treatment. The above results suggested that the estrogen upregulated the expression of NGF and ChAT. Furthermore, the similarity of their changing tendency implied that they were correlated and cooperated during the course in effect of estrogen on cerebellum. It also showed that the action of estrogen in cerebellum could via genomic and nongenomic mechanism.

罗启慧;陈正礼;范光丽. 雌激素对去卵巢大鼠小脑ER、ChAT、NGF表达的影响[J]. 畜牧兽医学报, 2007, 38(9): 959-965.

LUO Qi-hui;CHEN Zheng-li;FAN Guang-li. Effects of Estrogen on Expression of ER, NGF and ChAT in Cerebellum of Ovariectomized Female Sprague-Dawley Rat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2007, 38(9): 959-965.

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