Abstract: In the present study, doublelabeled immunohistochemistry were adopted to explore co-localization of gonadotropin releasing hormone (GnRH) and oxytocin (OT) in cells of hypothalamus of dairy goat during pregnancy. The results showed that neurons which were double positive with both antigens were localized in nuclei such as paraventricular nucleus, supraoptic nucleus, suprachiasmatic nucleus, arcuate nucleus, lateral hypothalamic area, medial mammillary nucleus and posterior mammillary nucleus.The number of these neurons was significantly changed during different period of gestation. These results provide morphological evidence for a direct modulation between GnRH and OT systems.