鸡传染性支气管炎病毒TJ9602、HN9604、BJ9601分离株特性的研究

畜牧兽医学报 ›› 2005, Vol. 36 ›› Issue (12): 1323-1328.doi:

鸡传染性支气管炎病毒TJ9602、HN9604、BJ9601分离株特性的研究

刘兴友;何宏轩;姚四新; 陈德胜; 甘孟侯; 陈溥言

1.南京农业大学，南京210095；2.河南科技学院，新乡453003；3.中国农业大学，北京 100094

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-12-25 发布日期:2005-12-25

Studies on the Character of Infectious Bronchitis Virus of TJ9602 Strain，HN9604 Strain and BJ9601 Strain Isolated from China

LIU Xing-you;HE Hong-xuan;YAO Si-xin;CHEN De-sheng;GAN Meng-hou;CHEN Pu-yan

1.Nanjing Agricultural University, Nanjing 210095,China; 2.Henan Institute of Science and Technology,Xinxiang 453003,China; 3.China Agricultural University, Beijing 100094 ,China

Received:1900-01-01 Revised:1900-01-01 Online:2005-12-25 Published:2005-12-25

摘要/Abstract

摘要： 从天津、河南、北京的鸡场分别分离到TJ9602、HN9604、BJ9601鸡传染性支气管炎病毒毒株，对其进行了动物回归、生物学特性、理化特性、血清学试验、抗原定位，对分离株的S1基因进行了RT-PCR扩增、核酸序列分析等研究。确定3株分离毒株为致肾病变的IBV，亲嗜器官为肾脏。HN9604和BJ9601与M41和H120均有较强的中和抗原交叉反应性，S1基因同源性分析，HN9604 和BJ9601 S1基因与H120毒株的同源性最高，与H120毒株同属于A分支，均属于Ⅰ群。 TJ9602与H120、M41等参考毒株的中和抗原交叉反应均较低，S1基因同源性分析，属于Ⅱ群。

Abstract: Three strains of infectious bronchitis virus were isolated from chicken farm in China, which were TJ9602 strain from Tianjin, HN9604 strain from Henan and BJ9601 strain from Beijing. Every virus strain were studied with chicken recurrent infection, biological characteristics assay, seroneutralization test, localization of IBV antigens, amplification of S1 gene and nucleic acid sequence analysis. The results showed that the three IBV strains were nephropathogenic strains and the target organs were the kidneys of chickens. HN9604 strain and BJ9601 strain shared highest homology of S1 gene sequence with H120 strain. HN9604, BJ9601, M41 and H120 can crossreact in seroneutralization test. In phylogenetic tree HN9604, BJ9601 were located in the cladogram embranchment A that includes H120 strain，they all belong to group Ⅰ. However, the TJ9602 strain is in another cladogram embranchment of group Ⅱ. It shared less homology of S1 gene sequence and shared weak crossreact in seroneutralization test with H120 strain or M41 strain.

刘兴友;何宏轩;姚四新;陈德胜;甘孟侯;陈溥言. 鸡传染性支气管炎病毒TJ9602、HN9604、BJ9601分离株特性的研究[J]. 畜牧兽医学报, 2005, 36(12): 1323-1328.

LIU Xing-you;HE Hong-xuan;YAO Si-xin;CHEN De-sheng;GAN Meng-hou;CHEN Pu-yan. Studies on the Character of Infectious Bronchitis Virus of TJ9602 Strain，HN9604 Strain and BJ9601 Strain Isolated from China[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2005, 36(12): 1323-1328.

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