Abstract: Porcine H-FABP gene expression diversity was investigated in cardiac and skeletal muscles of DLY(Duroc×Landrance×Yorkshire) and Ya’nan pigs weighed 100 kg by using RT-PCR.To collect the samples ,five pigs were slaughtered in each genotype. The results indicated that H-FABP gene expressed both in porcine cardiac and skeletal muscles. The expression in cardiac muscle was more active than in skeletal muscle (P<0.01). However, it was identified that H-FABP gene expression in Ya’nan pig was a little higher than in DLY both in cardiac and skeletal muscles(P>0.05).