Abstract: Three types of stalks from high oil corn variety (HOC 298), conventional corn (CAU3138) and specific fodder corn (FC3) were used to determine the alteration of main chemical contents and in vitro digestibility of HOC 298 stalks with kernel maturity stage and the difference of HOC stalks to other variety of corn stalks. The result showed that as the corn kernels matured, HOC298 stalks had linear increases (L; P<0.01) in the contents of water soluble carbohydrate (WSC), starch and ether extract (EE), and the digestibility of DM (DMD), but had a linear decrease (L; P<0.01) in the contents of NDF, ADF and lignin. The optimum harvest time for high oil corn HOC298 was, therefore, recommended at 4/4 milk line of kernels. The content of WSC, starch and EE of HOC298 stalks was not only much higher (P<0.01) than that of CAU3138 stalks harvested at 4/4 milk line, but also higher (P<0.06 or P<0.01) than that of FC3 stalks harvested at 3/4 milk line, whereas the content of cell walls including NDF, ADF and lignin of HOC 298 stalks was significantly lower (P<0.001) than that of CAU3138 and FC3, respectively. The stalks of HOC298 had higher (P<0.01) in vitro DMD and NDFD, and ruminal gas production than those of CAU3138 and FC3 stalks. These results suggested that HOC298 stalks harvested at 4/4 milk line stage of kernels are characterized by their high nutrient contents and being more ruminally digestible, and seem to be of highly valuable over other corn stalks.