Abstract: Applying simple after randomly sampling 18 Leiqiong cattle，19 Bayingole Menggu cattle and 18 Bayingole yak from typical colony in the central area of their habitats， the exon sequences (654 bp) of GH gene were analyzed by direct sequencing of PCR product. Together with homologous sequences of Bos taurus，Bos indicus，and Bos grunniens，using the sequence of Bubalus bubalis as the outgroup，the phylogenetic trees were constructed by NJ and MP methods respectively. The aim was to explore variation characteristics of GH gene，the relationships between southern and northern cattle of China and yak，and provide some considerable data for the evolution characteristics on Bovidae research and evaluate the relationship between adaptive evolution and molecular evolution research.The results showed that three mutations and five different haplotypes， two mutations and four different haplotypes，two mutations and three different haplotypes were detected in Leiqiong cattle，Bayingole Menggu cattle and Bayingole yak， respectively. Both three results supported almost the same topology，which indicated that GH gene differentiation happened before Bubalus bubalis differentiated from Bos indicus，Bos taurus and Bos grunniens. As to GH gene，the difference between yak and southern，northern cattle of China was not significant.