Abstract: The study was based on Jinding duck which was conserved in National Waterfowl Germplasm Resource Pool. The conserved effect was detected through comparing the difference of genetic diversity which was detected by 12 microsatellite markers in small populatin of three generations. The results suggested that 66 alleles were detected at 12 microsatellite loci in conserved population of three generations in Jinding duck. 60 alleles of the total were all detected in three generations， and 2 alleles were detected only in the first generation. The number of average alleles at each locus was more than 5. The mean heterozygosity of conserved population of three generations ranged from 0.712 9 to 0.729 8， and the mean polymorphism information content ranged from 0.665 8 to 0.687 1. The genetic diversity of Jinding duck was rich. The tendency was decrease following the raise of generation. The study showed that the small population conserved method in Jinding duck was feasible.