Abstract: The gene encoding immunoreactive epitops, residues 165 to 200 from the PCV2 capsid protein, was cloned into VP2 gene from porcine parvovirus. Then the recombinant porcine parvovirus-like particles(PPV-VLPs) was acquired by adenovirus vector system. After transfection， recombined virus (rAd-PPV∶VP2-PCV2∶Cap) was developed. The specific 64 kD band was showed by analysis of Western-blotting and specific fluorescence was observed by IFA which indicated that chimeric PPV VP2 protein had expressed in HEK-293 cells. Moreover， PPV:VP2-PCV2:Cap protein was to be similar to PPV in haemagglutinating activity. It was noteworthy that many self-assembled virus-like particles \[PPV:VLP(PCV2)\] were found in VP2 crude extracts by electronmicroscope.