猪IFN-γ和IL-4重组质粒对口蹄疫疫苗的免疫佐剂效应研究

畜牧兽医学报 ›› 2008, Vol. 39 ›› Issue (9): 1240-1244.doi:

猪IFN-γ和IL-4重组质粒对口蹄疫疫苗的免疫佐剂效应研究

蒙学莲，房永祥，窦永喜，陈国华，景志忠*，才学鹏*

中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室，兰州 730046

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-09-24 发布日期:2008-09-24
通讯作者: 景志忠，才学鹏

Immunological Adjuvant Effect of Recombinant Porcine IFN-γ and IL-4 Plasmid on Foot-and-mouth Disease Vaccine

MENG Xue-lian， FANG Yong-xiang， DOU Yong-xi， CHEN Guo-hua， JING Zhi-zhong*， CAI Xue-peng*

Key Laboratory of Veterinary Parasitology of Gansu Province， State Key Laboratory of Veterinary Etiological Biology， Lanzhou Veterinary Research Institute， Chinese Academy of Agricultural Sciences， Lanzhou 730046， China

Received:1900-01-01 Revised:1900-01-01 Online:2008-09-24 Published:2008-09-24
Contact: JING Zhi-zhong， CAI Xue-peng

摘要/Abstract

摘要： 为了研究猪IFN-γ和IL-4对疫苗的免疫佐剂效应，用pcDNA3.1/IFN-γ和pcDNA3.1/IL-4的重组质粒与口蹄疫双价疫苗配伍免疫小鼠，检测小鼠抗口蹄疫抗体水平和CD4+/CD8+T细胞比值的动态变化，观察其免疫佐剂效应。结果表明pcDNA3.1/IFN-γ和pcDNA3.1/IL-4重组质粒都能显著提高小鼠抗口蹄疫抗体水平(P<0.01)，其中pcDNA3.1/IFN-γ提高亚洲Ⅰ型FMD抗体水平的佐剂效应较pcDNA3.1/IL-4的显著(P<0.01)，而pcDNA3.1/IL-4提高O型FMD抗体水平的佐剂效应显著高于pcDNA3.1/IFN-γ(P <0.01)，pcDNA3.1也显示出了一定的佐剂效应；免疫后不同时间pcDNA3.1/IFN-γ和pcDNA3.1/IL-4组CD4⁺/CD8⁺T细胞比值显著高于其他组(P <0.01)，且第21天与第7、45天的差异极显著(P <0.01)。

Abstract: In order to certify the immunological adjuvant effect of recombinant pcDNA3.1/IFN-γ and pcDNA3.1/IL-4 plasmid， we investigated the changes of antibody levels and the CD4⁺/CD8⁺ relative value induced by a vaccine against foot-and-month disease and the mentioned adjuvants. The results showed that the recombinant plasmids significantly increased the level of antibody against foot-and-mouth disease (P<0.01)， and the adjuvant effect of pcDNA3.1/IFN-γ was much higher than that of pcDNA3.1/IL-4 concerning the antibody level of Serotype AsiaⅠ(P<0.01)，whereas the adjuvant effect of pcDNA3.1/IL-4 was more obvious than that of pcDNA3.1/IFN-γ concerning the antibody level of Serotype O(P<0.01). Relative values of CD4⁺/CD8⁺in groups pcDNA3.1/IFN-γ and pcDNA3.1/IL-4 were statistically significantly higher than that of the other groups(P<0.01)， and that on 21 d post immunization was statistically significantly higher than both on 7 and 45 d(P<0.01).

蒙学莲;房永祥;窦永喜;陈国华;景志忠;才学鹏. 猪IFN-γ和IL-4重组质粒对口蹄疫疫苗的免疫佐剂效应研究[J]. 畜牧兽医学报, 2008, 39(9): 1240-1244.

MENG Xue-lian;FANG Yong-xiang;DOU Yong-xi;CHEN Guo-hua;JING Zhi-zhong;CAI Xue-peng. Immunological Adjuvant Effect of Recombinant Porcine IFN-γ and IL-4 Plasmid on Foot-and-mouth Disease Vaccine [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2008, 39(9): 1240-1244.

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