Abstract: The expriment was designed to investigate the regulatory of digestion and metabolism of different dietary protein levels in mink at the period of the growth and development. Sixty young male minks were randomly divided into six groups according to a singlefactor test. The trials of feeding and metabolism were used to analyse the digestibility of protein, digestibility of fat, nitrogen deposition and protein biological value, combining with the ratio of feed conversion rate and the average daily gain to deduce the fluctuation regulation of nutrition in mink during the rearing process of the growth and development. The result showed that: the paremeters such as body weight, protein biological value and net protein utilization were significantly different among groups(P<0.05); feed conversion rate, digestibility of dry matters, digestibility of protein, digestibility of crude fat, intake nitrogen and urine nitrogen were greatly significantly different among minks (P<0.01); the more urine nitrogen was drained with the more dietary protein (P<0.05); the percentage of dietary protein was less than 32%, the feed conversion rate and average daily gain of minks were significantly reduced(P<0.05). The content of dietary protein was lower than 32%, the growth rate of mink would be obviously declined(P<0.05); the higher dietary protein level that over 34% promoted the digestibility of crude fat and dry matters;the optimum dietary protein level were 36% and 34% at 5080 day ages and 80110 day ages, respectively.