Abstract: To establish a method for effective isolation and amplification of Mongolia horse bonemarrow derived mesenchymal stem cells (MSCs) and verification by morphology and multiple differentiation potential. MSCs were isolated from Mongolia horse bone marrow by gradient centrifugation and different adherent time method. Morphology and growth characteristics were examined by phase contrast microscopy. MSCs were induced in vitro to adipocytes by dexamethasone, IBMX, insulin and rosiglitazone, and to osteoblasts by βglycerophosphate, Lascorbicacid2phosphate and dexamethasone. The result showed that primary and passage cells were spindleshaped and presented active proliferation. Cells were successfully induced to adipocytes and osteoblasts cells, and presented the characteristics of adipocytes and osteoblasts cells. The result indicate that the method is established to isolate and multiply MSCs from Mongolia horse bone marrow effectively and to verify by morphology and its multipotential differentiation. It prove that the purified cells are not adult somatic cells but matrix cells.