Abstract: The primers of 5′-regulatory region were designed according to chicken Myostatin gene. The fragment was amplified from goose genomic DNA, and cloned into pGEMT-Easy Vector and sequenced. A 1.3 kb long fragment of 5′regulatory region of goose Myostatin gene, which includes the putative initial transcriptional site, was acquired. The sequence homology analyses showed that the goose Myostatin 5′-regulatory region shared 94.8% with duck, 692% with chicken and 14.3% with mouse. Sequence analyses showed that there are a TATA box at －34 bp and a CCAAT box at －77 bp upstream of the putative transcriptional site. In addition, some muscle specific transcriptional factor binding sites including 5 E-boxes, 2 MEF2 binding sites, a MSX2 box and a muscle TATA box were determined. The Northern blot results in goose showed that Myostatin mRNA only expressed in breast muscle and thigh muscle and were not detectable in liver, heart, intestine, kidney, brain and lung.