鹅不同繁殖时期GnRH和GnIH基因表达和激素浓度变化分析

doi:doi: 10.11843/j.issn.0366-6964.2016.08.025

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (8): 1720-1726.doi: doi: 10.11843/j.issn.0366-6964.2016.08.025

鹅不同繁殖时期GnRH和GnIH基因表达和激素浓度变化分析

张克山^1，2，胡彦竞科³，韩笑哲³，高广亮^1，2，钟航^1，2，王启贵^1，2*

（1.重庆市畜牧科学院，重庆 402460；2.重庆市肉鹅遗传改良工程技术研究中心，重庆 402460；3.西南大学，重庆 402460）

收稿日期:2016-02-25 出版日期:2016-08-23 发布日期:2016-08-23
通讯作者: 王启贵，教授，博士，主要从事家禽遗传育种研究，E-mail: wangqigui@hotmail.com
作者简介:张克山（1988 ），女，四川达州人，助理研究员，硕士，主要从事家禽遗传育种研究，E-mail：zhangksh1988@163.com
基金资助:
重庆市基础科学与前沿研究(cstc2014jcyjA80021)；重庆市基本科研业务费(14305);国家自然科学基金（31572386）

Analysis of the Serum Concentrations and mRNA Expression Levels of GnRH and GnIH in Geese during Different Reproductive Periods

ZHANG Ke-shan ^1，2，HU Yan-jingke³，HAN Xiao-zhe ³，GAO Guang-liang ^1，2，ZHONG Hang^1，2，WANG Qi-gui ^1，2*

（1.Chongqing Academy of Animal Science,Chongqing 402460，China;
2.Chongqing Engineering Research Center of Goose Genetic Improvement,Chongqing 402460，China；3.Southwest University,Chongqing 402460，China）

Received:2016-02-25 Online:2016-08-23 Published:2016-08-23

摘要/Abstract

摘要：

旨在研究GnRH和GnIH基因和激素在鹅繁殖过程中的重要作用，本研究以产蛋性能差异显著的四川白鹅和溆浦鹅为试验材料，利用Real-time RCR方法检测产蛋前期、产蛋期和休产期两种鹅的下丘脑、垂体和卵巢组织中GnRH和GnIH mRNA的表达量；利用放射性免疫和酶联免疫吸附测定法测定血清中的GnRH和GnIH激素变化情况。结果表明：GnRH和GnIH基因在四川白鹅和溆浦鹅的下丘脑、垂体和卵巢组织中均有表达；产蛋前期和产蛋高峰期的下丘脑和卵巢组织中，四川白鹅的GnRH mRNA表达量显著（P<0.05）或极显著(P<0.01)高于溆浦鹅，在产蛋高峰期和休产期四川白鹅GnRH激素浓度也极显著(P <0.01)或显著高于（P<0.05）溆浦鹅，在高峰期和休产期四川白鹅GnRH血清浓度分别为51.13和51.10 pg•mL^-1，溆浦鹅分别为49.52和49.94 pg•mL^-1；GnIH在两种鹅的变化比较一致，仅在产蛋高峰期，四川白鹅下丘脑组织中GnIH mRNA表达水平极显著低于(P<0.01)溆浦鹅，而两种鹅之间的GnIH激素在各个时期均无显著差异。这提示随着繁殖阶段的改变，在调控两种鹅产蛋性能的作用过程中GnRH可能较GnIH 发挥着更为重要的作用，为进一步研究鹅繁殖分子机制奠定基础。

Abstract:

To investigate the role of gonadotropin-releasing hormone and gonadotropin-inhibitory hormone genes (GnRH and GnIH，respectively)during the reproductive process of geese，the Sichuan White goose and Xupu goose were selected as experimental animals.We detected the mRNA expression profiles of GnRH and GnIH in the hypothalamus，pituitary and ovary tissues using real-time reverse transcription PCR.Serum concentrations of GnRH and GnIH were respectively measured using radio immunoassay and ELISA during prelaying and laying periods and the period when laying ceased.GnIH and GnRH mRNA were expressed in the hypothalamus，pituitary and ovary tissues.GnRH mRNA expression of the hypothalamus and the ovary were significantly higher in Sichuan White geese than in Xupu geese in both prelaying and laying periods，particularly in the latter.Serum GnRH concentrations were significantly higher or higher in Sichuan White geese (51.13 and 51.10 pg•mL^-1) than in Xupu geese(49.52 and 49.94 pg•mL^-1) during the laying and cease period，respectively.During the laying period，GnIH mRNA expression of Sichuan White geese was significantly lower than in Xupu geese；however，there were no significant differences inserum GnIH concentrations between the 2 geese during prelaying，laying or cease periods.These results indicate that GnRH is more important than GnIH in regulating laying performance and changing the laying stages which establishes the foundation for understanding the breeding mechanisms of geese.

中图分类号:

835.2

张克山，胡彦竞科，韩笑哲，高广亮，钟航，王启贵. 鹅不同繁殖时期GnRH和GnIH基因表达和激素浓度变化分析[J]. 畜牧兽医学报, 2016, 47(8): 1720-1726.

ZHANG Ke-shan，HU Yan-jingke，HAN Xiao-zhe，GAO Guang-liang，ZHONG Hang，WANG Qi-gui. Analysis of the Serum Concentrations and mRNA Expression Levels of GnRH and GnIH in Geese during Different Reproductive Periods[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(8): 1720-1726.

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鹅不同繁殖时期GnRH和GnIH基因表达和激素浓度变化分析

Analysis of the Serum Concentrations and mRNA Expression Levels of GnRH and GnIH in Geese during Different Reproductive Periods

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