杜洛克猪与梅山猪副性腺组织学比较及精浆蛋白mRNA表达分析

doi:doi: 10.11843/j.issn.0366-6964.2016.08.024

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (8): 1714-1719.doi: doi: 10.11843/j.issn.0366-6964.2016.08.024

杜洛克猪与梅山猪副性腺组织学比较及精浆蛋白mRNA表达分析

丁海生1，徐德全1，2，3，罗琰1，刘敏1，2*

（1.华中农业大学动物科技学院动物医学院，武汉 430070；2.农业部猪遗传育种重点实验室，武汉 430070；3.生猪健康养殖协同创新中心，武汉 430070）

收稿日期:2016-01-29 出版日期:2016-08-23 发布日期:2016-08-23
通讯作者: 刘敏，博士，副教授，E-mail:liumin23@mail.hzau.edu.cn；Tel: 027-87282091
作者简介:丁海生（1987-），女，甘肃会宁人，博士生，主要从事动物分子生物学研究，E-mail: dinghs123@163.com
基金资助:
中央高校基本科研业务费专项资金（2662015PY014；2662015PY192；26620015PY108）；湖北省农业科技创新课题（2011-720）；国家科技支撑计划（2014BAD20B01）；国家自然科学基金（31201764）；973项目（2014CB138500）；湖北省自然科学基金（2015CFA101）；武汉市青年科技晨光计划（2014070404010204）

Histological Comparison and mRNA Expression Analysis of Porcine Seminal Protein on Accessory Glands of Duroc and Meishan Boars

DING Hai-sheng¹，XU De-quan^1，2，3，LUO Yan ¹，LIU Min ^1，2*

(1.College of Animal Science and Veterinary Medicine，Huazhong Agricultural University，Wuhan 430070，
China；2.Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry，Wuhan 430070，China；
3.The Cooperative Innovation Center for Sustainable Pig Production，Wuhan 430070，China)

Received:2016-01-29 Online:2016-08-23 Published:2016-08-23

摘要/Abstract

摘要：

旨在探讨杜洛克公猪和梅山公猪精囊腺和尿道球腺的组织学特征及精浆蛋白mRNA的表达，运用石蜡切片并通过HE染色，分别在光学显微镜下对75日龄、270日龄梅山公猪与270日龄杜洛克公猪的精囊腺和尿道球腺进行组织学观察；并用实时荧光定量PCR对精浆蛋白mRNA的表达进行分析。270日龄梅山公猪与75日龄梅山公猪相比，精囊腺的腺小叶增多，小叶间结缔组织减少；270日龄杜洛克公猪精囊腺较270日龄梅山公猪有更多的腺泡黏膜褶皱和小叶间结缔组织。梅山公猪和杜洛克公猪尿道球腺被结缔组织分隔成小叶，腺泡密集且腺腔小；梅山猪在成年时尿道球腺腺小叶增大，小叶间结缔组织变少；270日龄杜洛克公猪尿道球腺腺小叶比270日龄梅山公猪小，而腺泡较大，且结缔组织更多。精浆蛋白PSP-Ⅰ和PSP-Ⅱ在精囊腺中高表达，在成熟的杜洛克和梅山公猪精囊腺中其mRNA表达水平相近(P＞0.05)。但随着日龄的增长，梅山公猪PSP-Ⅰ和PSP-Ⅱ mRNA表达水平显著升高(P＜0.05)。杜洛克公猪更多的精囊腺黏膜褶皱和更大的尿道球腺腺泡，增加了上皮分泌面积，有助于增加精浆体积，提高精子代谢率和存活率。另外，性成熟公猪中精浆蛋白PSP-Ⅰ和PSP-Ⅱ的高表达可能参与维持精子的代谢和活力等生理过程。

Abstract:

In order to assess the histological features of the vesicular gland and bulbourethral gland，and the mRNA expression of porcine seminal protein in 270-day-old Duroc and 75-，270- day-old Meishan boars，the paraffin sections with HE staining were performed and observed under light microscope，and quantitative real-time PCR was also used.Increased glandular lobules and decreased interlobular connective tissues were found in the vesicular glands of 270-day-old Meishan boars compared to those of 75-day-old Meishan boars.More acinus mucosal folds and interlobular connective tissues were found in the vesicular glands of 270-day-old Duroc boars than those of 270-day-old Meishan boars.The bulbourethral glands were composed of multiple lobules separated by connective tissue，and dense acini with small lumens were found.When Meishan boars reached adult age，the enlarged glandular lobules and decreased interlobular connective tissues were observed in the bulbourethral glands.In addition，larger glandular lobules，smaller acini and less interlobular connective tissues were observed in the bulbourethral glands of 270-day-old Meishan boars than those of 270-day-old Duroc boars.PSP-Ⅰ and PSP-Ⅱ were expressed highly in seminal vesicles，but there was no significant difference between the mature Duroc and Meishan boars (P>0.05).With advancing age，the mRNA expression level of PSP-Ⅰ and PSP-Ⅱ increased significantly in Meishan boars (P<0.05).Overall，more acinus mucosal folds in vesicular glands and larger acini in the bulbourethral glands of Duroc boars increased the epithelial surface area available for secretion and led to an increase in the semen volume and the rate of sperm metabolism and survival.Moreover，high expression of PSP-Ⅰ and PSP-Ⅱ in mature boars may play a role in some physiological processes，such as maintaining sperm metabolism and motility.

中图分类号:

S852.16+2

丁海生，徐德全，罗琰，刘敏. 杜洛克猪与梅山猪副性腺组织学比较及精浆蛋白mRNA表达分析[J]. 畜牧兽医学报, 2016, 47(8): 1714-1719.

DING Hai-sheng，XU De-quan，LUO Yan，LIU Min. Histological Comparison and mRNA Expression Analysis of Porcine Seminal Protein on Accessory Glands of Duroc and Meishan Boars[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(8): 1714-1719.

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杜洛克猪与梅山猪副性腺组织学比较及精浆蛋白mRNA表达分析

Histological Comparison and mRNA Expression Analysis of Porcine Seminal Protein on Accessory Glands of Duroc and Meishan Boars

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