湘西黄牛Myf5和Pax7基因的SNPs检测及其与体尺性状的关联分析

doi:10.11843/j.issn.0366-6964.2014.09.021

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (9): 1531-1537.doi: 10.11843/j.issn.0366-6964.2014.09.021

湘西黄牛Myf5和Pax7基因的SNPs检测及其与体尺性状的关联分析

王兴平^*，罗仍卓么，李峰，李德才，都鑫

(湖南文理学院生命科学学院，湖南省高校动物学重点实验室，常德 415000)

收稿日期:2014-03-04 出版日期:2014-09-23 发布日期:2014-09-23
通讯作者: 王兴平，E-mail：huaswxp@hotmail.com
作者简介:王兴平（1978-），男，陕西宝鸡人，博士，副教授，主要从事动物基因表达调控与分子育种研究
基金资助:
湖南省科技计划项目(2010NK3043)；湖南文理学院博士启动项目(BSQD1002)；湖南省动物学重点学科建设项目

Identification of SNPs in Myf5 and Pax7 Genes and Association Analysis with Body Measurement Traits in Xiangxi Cattle

WANG Xing-ping^*，LUORENG Zhuo-ma,LI Feng，LI De-cai，DU Xin

(Key Laboratory of Zoology in Hunan Higher Education School，College of Life Science，Hunan University of Arts and Science，Changde 415000，China)

Received:2014-03-04 Online:2014-09-23 Published:2014-09-23

摘要/Abstract

摘要：

本研究旨在检测湘西黄牛Myf5和Pax7基因的单核苷酸多态性(SNP)，探讨湘西黄牛多态性位点的群体遗传特征，寻找与生长发育相关的分子标记。采用PCR和直接测序技术筛查湘西黄牛Myf5基因第2内含子和Pax7基因第3外显子的SNPs；以217头湘西黄牛为研究对象，采用PCR-RFLP方法对群体进行遗传多态性分析，运用最小二乘线性模型进行基因型与体尺性状的关联分析。结果表明，在湘西黄牛Myf5基因第2内含子中检测到9个SNPs，其中7个是本研究新发现的SNPs；在Pax7基因第3外显子检测到1个SNP。群体遗传多态性分析结果显示，湘西黄牛群体中Myf5基因g.1948A>G位点的AA、AG和GG基因型频率分别为0.046 1、0.235 0和0.718 9；A和G等位基因频率分别为0.163 6和0.836 4。Pax7基因g.31G>A位点只检测到AG和GG 2种基因型，基因型频率分别为0.073 7和0.926 3；A和G等位基因频率分别为0.036 9和0.963 1。这2个位点均处于低度多态，且达到了Hardy-Weinberg平衡状态(P>0.05)。关联分析结果表明，Myf5基因g.1948A>G位点的AA和AG型个体的体重显著高于GG型个体的体重(P<0.05)，A等位基因对体重为正效应；而各基因型间的体高、体长和胸围的差异均不显著(P>0.05)。结果提示，湘西黄牛Myf5基因的SNP位点较多，g.1948A>G位点可能为湘西黄牛体重性状的分子遗传标记位点。

Abstract:

This research aimed to identify single nucleotide polymorphism (SNP) of Myf5 and Pax7 genes in Xiangxi cattle，investigate the population genetic characters of SNPs and search the molecular markers for growth.SNPs of intron 2 in Myf5 gene and exon 3 in Pax7 gene were detected by PCR and sequencing methods.Population genetic polymorphisms for 217 Xiangxi cattle were detected by PCR-RFLP，and the association analysis between genotypes and body measurement traits was performed by least-squares linear model.The results showed that the total of 9 SNPs were detected on intron 2 in Myf5 gene，in which，7 SNPs were novel.While，only one SNP was detected on exon 3 in Pax7 gene.The results of population genetic polymorphism indicated，for g.1948A>G locus of Myf5 gene in Xiangxi cattle，the genotype frequencies of AA，AG，GG were 0.046 1，0.235 0，0.718 9.Allele frequencies of A and G were 0.163 6 and 0.836 4，respectively.For g.31G>A locus of Pax7 gene，only two genotypes of AG and GG were detected in Xiangxi cattle，and the genotype frequencies were 0.073 7 and 0.926 3，the allele frequencies of A and G were 0.036 9 and 0.963 1，respectively.Both loci were low polymorphism，and fitted with Hardy-Weinberg equilibrium (P>0.05).The results of association analysis for g.1948A>G locus in Myf5 gene revealed that the animals with AA and AG genotypes had higher body weight than those with GG genotype in Xiangxi cattle (P<0.05)，while there were not significant differences for withers height，body length and heart girth among the individuals with different genotypes (P>0.05).Allele A positively affected on body weight in Xiangxi cattle.These results indicated that there were 9 SNPs in Myf5 gene，in which，g.1948A>G locus might be molecular marker for body weight.

中图分类号:

王兴平，罗仍卓么，李峰，李德才，都鑫. 湘西黄牛Myf5和Pax7基因的SNPs检测及其与体尺性状的关联分析[J]. 畜牧兽医学报, 2014, 45(9): 1531-1537.

WANG Xing-ping，LUORENG Zhuo-ma,LI Feng，LI De-cai，DU Xin. Identification of SNPs in Myf5 and Pax7 Genes and Association Analysis with Body Measurement Traits in Xiangxi Cattle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(9): 1531-1537.

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湘西黄牛Myf5和Pax7基因的SNPs检测及其与体尺性状的关联分析

Identification of SNPs in Myf5 and Pax7 Genes and Association Analysis with Body Measurement Traits in Xiangxi Cattle

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