畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (3): 410-416.doi: 10.11843/j.issn.0366-6964.2014.03.010

• 动物营养 • 上一篇    下一篇

锌、维生素E和β-胡萝卜素对种公牛睾酮、细胞因子及免疫性能的影响

李秋凤1,2,3,李建国1,2,3*,高艳霞1,2,3,曹玉凤1,2,3,李运起1,2,3,冯志华1,2,3,马亚斌4,吴云海4,刘兴华4   

  1. (1.河北农业大学动物科技学院,保定 071001; 2.河北省牛羊胚胎工程技术研究中心,保定 071001;3.河北农业大学 养牛科学研究所,保定 071001; 4.河北省畜牧良种工作站,石家庄 050061)
  • 收稿日期:2013-09-14 出版日期:2014-03-23 发布日期:2014-03-23
  • 通讯作者: 李建国,教授,博导,E-mail:jgli@hebau.edu.cn
  • 作者简介:李秋凤(1975-),女,河北乐亭人,博士,副教授,主要从事反刍动物营养与饲料科学研究,E-mail:lqf582@126.com
  • 基金资助:

    国家现代农业(奶牛)产业技术体系建设专项资金(CARS-37);国家现代农业(肉牛)产业技术体系建设专项资金(CARS-38)

The Effects of Vitamin E,Beta-carotene and Zinc on Testosterone,Cytokine and Immune Function of Holstein Bull in Hot Weather

LI Qiu-feng1,2,3,LI Jian-guo1,2,3*,GAO Yan-xia1,2,3,CAO Yu-feng1,2,3,LI Yun-qi1,2,3,FENG Zhi-hua1,2,3,MA Ya-bin4,WU Yun-hai4,LIU Xing-hua4   

  1. (1.College of Animal Science and Technology,Agricultural University of Hebei,Baoding 071001, China;2.Embryo Engineering and Technological Center of Cattle and Sheep of Hebei,Baoding 071001, China;3.Cattle Science Institute,Agricultural University of Hebei,Baoding 071001, China;4.Animal Husbandry Workstation of Hebei Province,Shijiazhuang 050061, China)
  • Received:2013-09-14 Online:2014-03-23 Published:2014-03-23

摘要:

通过研究高温季节日粮中添加维生素(维生素E和β-胡萝卜素)和锌对荷斯坦公牛血清和精清中睾酮、铜蓝蛋白、细胞因子的变化,探讨高温季节维生素和锌对公牛繁殖性能的影响机制。本试验采用随机区组设计,选取体重相近(1 010±80)kg、采精正常、健康无病的纯种荷斯坦成年种公牛60头,随机分为4组,每组15头。4个处理组分别为Ⅰ组:对照组(基础日粮);Ⅱ组:基础日粮+100 mg·kg-1 DM Zn;Ⅲ组:基础日粮+维生素(300 mg·kg-1DM维生素E和60 mg·kg-1DM β-胡萝卜素);Ⅳ组:基础日粮+100 mg·kg-1DM Zn+维生素(300 mg·kg-1DM维生素E和60 mg·kg-1DM β-胡萝卜素),试验期120 d。结果表明:血清中睾酮浓度随着维生素(P<0.05)和锌(P<0.01)单独添加而显著增加,二者共同添加显著增加睾酮的浓度(P<0.01),精清中睾酮的浓度以Ⅲ组(添加维生素)最高(P<0.05),其次为Ⅳ组和Ⅱ组。日粮中添加锌显著增加血清(P<0.01)和精清(P<0.05)中铜蓝蛋白的浓度;单独添加维生素对血清和精清中铜蓝蛋白的浓度无显著影响,当锌和维生素共同添加可显著增加血清(P<0.01)和精清(P<0.01)中铜蓝蛋白的浓度。日粮中添加维生素和锌显著降低血清中IL-6(P<0.01)、IL-17(P<0.01)和TNF-α(P<0.05)的浓度,降低精清中IL-6的浓度(P<0.01)。综上可见,日粮中添加锌和维生素可提高精子的抗氧化能力。

Abstract:

The test was to study the effects of dietary vitamins (vitamin E and beta-carotene) and zinc on semen quality,antioxidant indicators of Holstein bull in hot weather.Sixty healthy and purebred Holstein bulls (BW=1 010±80 kg) were randomly classified into 4 groups (n=15):group Ⅰ,control group (basal diet); group II,basal diet +100 mg·kg-1 DM Zn; group III,basal diet+vitamins (300 mg·kg-1 DM vitamin E and 60 mg·kg-1 DM beta-carotene) and group IV,basal diet +100 mg·kg-1 DM Zn + vitamins (300 mg·kg-1 DM vitamin E and 60 mg·kg-1 DM beta-carotene).The trial duration was 120 days.The results showed that the serum concentration of testosterone increased significantly with the addition of vitamin (P<0.05) and zinc (P<0.01),the concentration of testosterone in seminal highest (P<0.05) in group Ⅲ(vitamins),followed by group ⅣandⅡ.Dietary zinc significantly increased serum (P<0.01) and seminal (P<0.05) concentrations of ceruloplasmin;there was no significant effect on serum concentration and seminal ceruloplasmin in group Ⅲ,when addition of the zinc and vitamin together could significantly improve serum (P<0.01) and seminal (P<0.01) concentrations of ceruloplasmin.Serum IL-6 (P<0.01), IL-17 (P<0.01) and TNF-α (P<0.05) concentrations as well as the seminal IL-6 (P<0.01) concentrations decreased significantly,which were caused by the supplemental vitamins and zinc.Dietary zinc and vitamins could increase the antioxidant capacity of sperm.

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