蒙古羊ADAMTS1基因外显子3多态性检测及在卵巢和子宫组织中差异表达分析

畜牧兽医学报

蒙古羊ADAMTS1基因外显子3多态性检测及在卵巢和子宫组织中差异表达分析

何小龙^1，2，李蓓³，刘永斌^2*，祁云霞^1,2，吴江鸿^1，2，特日格勒²，荣威恒²

（1. 中国科学院内蒙古草业研究中心，呼和浩特 010031； 2. 内蒙古自治区农牧业科学院，呼和浩特 010031； 3. 内蒙古农业大学动物科学学院，呼和浩特 010018）

收稿日期:2012-09-21 出版日期:2013-03-23 发布日期:2013-03-23
通讯作者: 刘永斌，研究员，E-mail: ybliu117@126.com
作者简介:何小龙(1983-)，男，陕西宝鸡眉县人，博士，主要从事生物技术与草食家畜育种研究，E-mail: hexiaolong1983@163.com
基金资助:
内蒙古自治区自然科学基金博士基金项目（2011BS0406）；内蒙古自治区农牧业科学院青年创新基金项目(2011QNJJM01)；国家肉羊产业技术体系（CARS-39）

The Polymorphism Detection of Exon 3 and Differential Expression Analysis in Ovaries and Uterus of Mongolian Sheep ADAMTS1 Gene

HE Xiao-long^1,2, LI Bei³, LIU Yong-bin^2*, QI Yun-xia^1,2, WU Jiang-hong^1,2, TE Ri-ge-le², RONG Wei-heng²

(1. Inner Mongolia Prataculture Research Center, Chinese Academy of Sciences, Huhhot 010031,China; 2. Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Huhhot 010031,China; 3. College of Animal Science, Inner Mongolia Agricultural University, Huhhot 010018,China)

Received:2012-09-21 Online:2013-03-23 Published:2013-03-23

摘要/Abstract

摘要：

为揭示蒙古羊卵巢组织差异表达基因ADAMTS1外显子3的遗传多态性及在单、双羔蒙古羊卵巢和子宫组织中的表达差异性，本试验分别采用PCR-SSCP技术结合DNA直接测序技术对蒙古羊ADAMTS1基因外显子3的遗传多态位点进行了检测；采用实时荧光定量PCR（RQ-PCR）技术对蒙古羊ADAMTS1基因在单、双羔蒙古羊卵巢和子宫组织中的差异表达量进行了分析。结果表明，蒙古羊ADAMTS1基因的第3外显子第141碱基处存在C→T的点突变，而该处突变未能引起氨基酸序列的变化，χ²适合性检验结果表明整个蒙古羊群体呈现低度多态（PIC=0.233）并处于Hardy-Weinberg非平衡状态（P<0.05）；RQ-PCR检测结果表明ADAMTS1基因在双羔羊卵巢组织和子宫组织中的表达量均高于单羔羊，分别是单羔羊相应组织表达量的2.04和2.30倍。结果表明，ADAMTS1基因对于蒙古羊的多羔性状具有重要的作用，是蒙古羊多羔主效基因。

Abstract:

This experiment was conducted to reveal the polymorphism of exon 3 of ADAMTS1 gene which was differentially expressed between Mongolian sheep (MS) ovaries, and to analyze the different expression in single-bearing and biparous MS ovaries and uterus tissue. The polymorphism of ADAMTS1 gene in exon 3 was detected by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and direct DNA sequencing technique, and differential expression in single-bearing and biparous MS ovaries and uterus tissue was detected by real-time quantitative PCR (RQ-PCR). The result showed that the ADAMTS1 gene had a C→T point mutation for the base 141 in exon 3 coding region, but not caused the amino acid sequence changes. The results of χ² fitness test indicated that all the MS populations in low polymorphism (PIC=0.233) and not in Hardy-Weinberg equilibrium (P<0.05). The expression of ADAMTS1 gene in biparous MS ovaries and uterus tissue was 2.04 and 2.30-fold that of single-bearing MS by using RQ-PCR. The results indicated that ADAMTS1 gene was the major gene and played an important role to the MS fecundity traits.

中图分类号:

S826.8⁺2

何小龙，李蓓，刘永斌，祁云霞，吴江鸿，特日格勒，荣威恒. 蒙古羊ADAMTS1基因外显子3多态性检测及在卵巢和子宫组织中差异表达分析[J]. 畜牧兽医学报, doi: .

HE Xiao-long, LI Bei, LIU Yong-bin, QI Yun-xia, WU Jiang-hong, TE Ri-ge-le, RONG Wei-heng. The Polymorphism Detection of Exon 3 and Differential Expression Analysis in Ovaries and Uterus of Mongolian Sheep ADAMTS1 Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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