Abstract: Ducks were intranasal immunized with inactivated avian influenza H9N2 virus (IAIV) in combination with sodium taurodeoxycholate hydrate and CpG DNA. Distribution and number of antibody secreting cells in duck respiratory tract were studied. Results showed that 3, 5 and 7 weeks after vaccination with sodium taurodeoxycholate hydrate and CpG DNA, the area of IgA and IgG secreting cells of nasal cavity, trachea and tracheal bifurcation were increased significantly （P<0.05 or P<0.01） compared with that of ducks with IAIV alone. After intranasal immunization with IAIV and CpG DNA, the area of IgA and IgG secreting cells of nasal cavity and trachea were partly increased (P<0.05). While no significant change on the area of IgA and IgG antibody secreting cells of respiratory tract tissues was observed in respiratory tract between ducks with IAIV alone and PBS. It demonstrated that intranasal immunization with IAIV combined with sodium taurodeoxycholate hydrate and CpG DNA could increase the area of IgA antibody secreting cells and IgG antibody secreting cells and enhance the level of local humoral immune response of duck’s respiratory tract.