谷氨酰胺对AA肉鸡生产性能、肌肉品质及H-FABP基因表达的影响

畜牧兽医学报

谷氨酰胺对AA肉鸡生产性能、肌肉品质及H-FABP基因表达的影响

钟金凤^1,2，胡永灵^1*，杨永生²，罗佳杰²

(1. 湖南环境生物职业技术学院，衡阳 421005； 2. 湖南农业大学大学动物科技学院，长沙 410128)

收稿日期:2012-05-08 出版日期:2012-11-26 发布日期:2012-11-26
通讯作者: 胡永灵,E-mail: 962567227@qq.com
作者简介:钟金凤(1980-)，女，四川南充人，讲师，博士生，主要从事动物生态营养学研究，E-mail:498379002@qq.com
基金资助:
教育部、财政部支持的国家重点专业（教职成厅函2011［71］号）；湖南省青年骨干教师资助（湘教通［2010］345号）

Effect of Glutamine on Growth Performance, Meat Quality and Expression Level of H-FABP of AA Broilers

ZHONG Jin-feng^1,2, HU Yong-ling^1*, YANG Yong-sheng², LUO Jia-jie²

(1. Hunan Polytechnic of Environment and Biology, Hengyang 421005, China; 2. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China)

Received:2012-05-08 Online:2012-11-26 Published:2012-11-26

摘要/Abstract

摘要： 旨在研究日粮中外源添加谷氨酰胺（Glutamine, Glu）对AA肉公鸡生产性能、胴体性状、肌肉品质、肌苷酸含量和H-FABP mRNA表达水平的影响。采用单因子完全随机设计，将240只1 d的雄性AA肉仔鸡随机均分为4组（A、B、C和D），分别在基础日粮中添加0、0.2%、0.4%和0.8%水平的谷氨酰胺，21和42 d时测定并计算日增体质量、日采食量和料重比；42 d时屠宰，测定其剪切力、滴水损失和色度（L^*、a^*和b^*）；计算屠宰率、全净膛率、半净膛率、胸肌率、腿肌率和腹脂率；取胸肌和腿肌，用高效液相色谱（HPLC）法测定肌苷酸含量，利用RT-PCR技术检测HFABP mRNA表达水平的变化。B组可显著提高AA肉鸡的平均日增体质量和平均日采食量（P<0.05），显著增加胸肌、腿肌的肌苷酸含量和H-FABP mRNA的表达水平（P<0.05）；C组可显著增加全净膛率、腿肌率、肌间脂肪宽度、胸肌剪切力和减少腹脂率（P＜0.05）；D组可显著增加腿肌剪切力、胸肌和腿肌的L^*和a^*值（P＜0.05）。日粮中添加0.2%、0.4%和0.8%水平的Glu均可不同程度提高肉鸡生产性能、胴体性状、肌肉品质、肌苷酸的含量和H-FABP mRNA表达水平，在生产中0.2%水平为建议添加量。

Abstract: The experiment was conducted to determine the effect of exogenous glutamine on the growth performance, carcass traits, meat quality, the content of inosine monophosphate and the expression level of H-FABP in breast and thigh muscle of AA male broilers. A total of 240 AA male broilers were randomly divided into 4 groups (A, B, C and D) which were fed with basic diet supplemented with Glu at 0, 0.2%, 0.4% and 0.8% level, respectively, the average daily gain(ADG), average daily feed intake(ADFI) and feed/gain ratio(F/G) were determined at 21^st and 42^nd day, and the experiment lasted for 42 days. The shear force, drip loss and chromal(L^*, a^*and b^*) were determined and the dressing percentage, eviscerated yield, half eviscerated yield, percentage of breast and thigh muscle were calculated at 42^nd day. The content of inosine monophosphate was detected by HPLC, and RT-PCR was used to determine the expression level of H-FABP mRNA in breast and thigh muscle. The average daily gain, average daily intake, the content of inosine monophosphate and the expression level of H-FABP mRNA of broilers were improved significantly in group B(P<0.05). The eviscerated yield, percentage of thigh muscle, the breadth of subcutaneous fat, shear force of breast were increased and percentage of abdominal fat were decreased significantly in group C (P<0.05). The shear force of thigh, the L^* and a^*value of breast and thigh muscle were enhanced significantly in group D (P<0.05). The result indicate that Glu supplement at 0.2%, 0.4% and 0.8% levels could improve the growth performance, carcass traits and meat quality of broilers effectively, and the contents of inosine monophosphate and expression level of H-FABP mRNA in breast and thigh muscle could be increased to some extent. The 0.2% level is the recommended dosage in the production.

中图分类号:

S852.4

钟金凤，胡永灵，杨永生，罗佳杰. 谷氨酰胺对AA肉鸡生产性能、肌肉品质及H-FABP基因表达的影响[J]. 畜牧兽医学报, doi: .

ZHONG Jin-feng, HU Yong-ling, YANG Yong-sheng, LUO Jia-jie. Effect of Glutamine on Growth Performance, Meat Quality and Expression Level of H-FABP of AA Broilers[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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