猪红细胞生成素受体基因突变与血常规性状关联分析

畜牧兽医学报

猪红细胞生成素受体基因突变与血常规性状关联分析

宋欣^1，2，曹随忠²，张龙超^1*，程笃学¹，王立刚¹，李勇¹，刘欣¹，马小军¹，梁晶¹，陈少康³，罗维真¹，颜华¹，赵克斌¹，王立贤^1*

（1.中国农业科学院北京畜牧兽医研究所，北京 100193； 2.四川农业大学动物医学院，雅安 625014； 3.中国农业大学动物科技学院，北京 100193）

收稿日期:2012-02-09 出版日期:2012-11-26 发布日期:2012-11-26
通讯作者: 张龙超，E-mail: lczhang@iascaas.net.cn；王立贤，E-mail：iaswlx@263.net
作者简介:宋欣（1986-），男，河南虞城人，硕士生，主要从事兽医产科学方面的研究，E-mail：songxin7788@163.com
基金资助:
“十二五”国家科技支撑计划项目（2011BAD28B01）；现代农业产业技术体系；中国农业科学院基本科研业务专项（2011cj-5）

Association of SNPs in Erythropoietin Receptor Gene with Blood Parameters

SONG Xin^1,2, CAO Sui-zhong², ZHANG Long-chao^1*, CHENG Du-xue¹,WANG Li-gang¹，LI Yong¹，LIU Xin¹，MA Xiao-jun¹，LIANG Jing¹,CHEN Shao-kang³，LUO Wei-zhen¹, YAN Hua¹, ZHAO Ke-bin¹, WANG Li-xian^1*

(1. Institute of Animal Science, Chinese Academy of Agricultural Scineces, Beijing 100193, China; 2. College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, China; 3. College of Animal Science and Technology,China Agricultural University, Beijing 100193, China)

Received:2012-02-09 Online:2012-11-26 Published:2012-11-26

摘要/Abstract

摘要： 本研究旨在探索红细胞生成素受体基因（EPOR）影响红细胞相关性状的分子机理。对EPOR基因第1内含子和第4内含子突变位点g.705G>T和g.2373C>T，以飞行时间质谱进行个体基因型分型，在构建的大白猪×民猪F₂代资源群体内开展其与6种血常规性状（红细胞压积、血红蛋白、平均红细胞血红蛋白量、平均红细胞体积、红细胞计数及红细胞分布宽度）的关联分析。结果表明，第1内含子突变位点g.705G>T未发现与血常规性状显著关联；第4内含子突变位点g.2373C>T群体内只发现2种基因型，其中CT基因型个体的红细胞压积显著高于CC基因型个体（P<0.05），但并未发现与其他性状显著关联。结果显示，EPOR基因突变可显著影响红细胞压积，说明该基因可作为影响红细胞压积的主效候选基因开展深入研究。

Abstract: The aim of present study was to investigate the effect of porcine erythropoietin receptor (EPOR) gene on blood parameters. Six blood parameters, hematocrit (HCT), hemoglobin (HGB), mean corpuscular hemoglobin (MCH), mean cell volume (MCV), red blood cell count (RBC) and red blood cell distribution width (RDW), were measured in Large White × Minzhu intercross porcine population. EPOR was genotyped using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF). The associations of g.705G>T in intron 1 and g.2373C>T in intron 4 with the six blood parameters were analyzed. The result showed that the SNP at g.705G>T was not significantly associated with any trait. Two genotypes (CC and CT) of SNP at g.2373C>T were found in this population. The HCT of individuals with CT genotype was higher than that of individuals with CC genotype (P<0.05), while this SNP was not significant associated with other five traits. These results indicate that the mutation of EPOR gene can affect the HCT and this gene can be a potential candidate gene for immunity relate traits (e.g. HCT) for further study.

中图分类号:

S828
S813.3

宋欣，曹随忠，张龙超，等. 猪红细胞生成素受体基因突变与血常规性状关联分析[J]. 畜牧兽医学报, doi: .

SONG Xin, CAO Sui-zhong, ZHANG Long-chao,et al. Association of SNPs in Erythropoietin Receptor Gene with Blood Parameters[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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