小型猪特异性麻醉颉颃剂对XFM麻醉下大鼠海马c-jun基因mRNA转录的影响

畜牧兽医学报

小型猪特异性麻醉颉颃剂对XFM麻醉下大鼠海马c-jun基因mRNA转录的影响

李小波¹, 尹柏双^1,2, 李志强¹, 石星星¹, 范宏刚¹, 王洪斌^1*

（1. 东北农业大学动物医学学院，哈尔滨 150030； 2. 吉林农业科技学院动物医学学院，吉林 132101 ）

收稿日期:2012-03-19 出版日期:2012-10-25 发布日期:2012-10-25
通讯作者: 王洪斌，E-mail：hbwang@neau.edu.cn
作者简介:李小波（1987-），男，四川南部人，硕士生，主要从事于动物麻醉与镇痛方面的研究， E-mail：libo870304boy@tom.com
基金资助:
国家自然科学基金项目( 30671552; 30972227)

Effects of the Specificity Antagonist for Miniature Pigs Combined Anaesthetic on the Transcription of c-jun Gene mRNA in the Hippocampus of Rats Anesthetized with XFM

LI Xiao-bo¹, YIN Bai-shuang^{1, 2}, LI Zhi-qiang¹, SHI Xing-xing¹, FAN Hong-gang¹, WANG Hong-bin^1*

（1. College of Animal Medicine, Northeast Agricultural University, Harbin 150030, China;
2. Department of Animal Medicine, Jilin Agricultural Science and Technology College, Jilin 132101, China）

Received:2012-03-19 Online:2012-10-25 Published:2012-10-25

摘要/Abstract

摘要： 本试验旨在研究小型猪特异性麻醉颉颃剂对小型猪特异性麻醉剂（XFM）麻醉下大鼠海马脑区c-jun基因mRNA转录的影响，藉以探究其催醒机制。90只成年SD大鼠被随机分成XFM麻醉组、XFM麻醉+生理盐水组、XFM麻醉+小型猪特异性麻醉颉颃剂组，每组分为5个不同采样时间点的亚组。采用实时荧光定量PCR技术检测样品中c-jun基因mRNA转录量。结果显示，XFM麻醉诱导大鼠海马脑区c-jun基因mRNA转录，各时间点海马脑区c-jun基因mRNA转录与0 min比较显著升高（P<0.01），XFM麻醉大鼠腹腔注射生理盐水后各时间点海马脑区c-jun基因mRNA转录与XFM对照组间无显著差异（P>0.05），注射小型猪特异性麻醉颉颃剂后抑制了XFM诱导大鼠海马c-jun基因mRNA的转录，与XFM对照组比较差异显著（P<0.01或P<0.05）。结果表明，小型猪特异性麻醉颉颃剂抑制了XFM麻醉诱导大鼠海马脑区c-jun基因mRNA的转录，这可能与小型猪特异性麻醉颉颃剂催醒机制相关。

Abstract: To explore the antagonism mechanism of the specificity antagonist for miniature pigs combined anaesthetic, the effect of the specificity antagonist on the c-jun gene transcription in the hippocampus of rats anesthetized with XFM was studied in this experiment. Ninety adult SD rats were divided randomly into three groups: XFM group, XFM add saline group and XFM add the specificity antagonist group. Each group consists of five subgroups, and samples were collected at different time points. The transcription of c-jun gene mRNA in every sample was detected with real time PCR. The result showed that The c-jun gene mRNA contents in each time point of the XFM group were obviously increased as compared with 0 min (P<0.01). There were no significant effect of injection of saline after XFM administrated on the c-jun gene mRNA transcription (P>0.05). The level of c-jun gene mRNA transcription after the specificity antagonist administrated was significantly lower than XFM group (P<0.01 or P<0.05). These results indicated that the specificity antagonist for miniature pigs combined anaesthetic attenuates the c-jun gene mRNA transcription in the hippocampus of rats induced by XFM, this may be related to the antagonism mechanism of the specificity antagonist for miniature pigs’ combined Anaesthetic.

中图分类号:

S8529.791

李小波, 尹柏双, 李志强, 石星星, 范宏刚, 王洪斌. 小型猪特异性麻醉颉颃剂对XFM麻醉下大鼠海马c-jun基因mRNA转录的影响[J]. 畜牧兽医学报, doi: .

LI Xiao-bo, YIN Bai-shuang, LI Zhi-qiang, SHI Xing-xing, FAN Hong-gang, WANG Hong-bin. Effects of the Specificity Antagonist for Miniature Pigs Combined Anaesthetic on the Transcription of c-jun Gene mRNA in the Hippocampus of Rats Anesthetized with XFM[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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