猪链球菌-副猪嗜血杆菌二联亚单位疫苗在小鼠的免疫效力分析

畜牧兽医学报

猪链球菌-副猪嗜血杆菌二联亚单位疫苗在小鼠的免疫效力分析

赵建平，魏燕鸣，宋岱松，张强，朱剑，周明光，李冉，康超，金梅林^*

（华中农业大学农业微生物国家重点实验室，武汉 430070）

收稿日期:2012-02-16 出版日期:2012-10-25 发布日期:2012-10-25
通讯作者: 金梅林，教授，E-mail：jml8328@126.com. Tel：027-87282608
作者简介:赵建平（1986-），女，江苏连云港人，硕士，主要从事动物细菌性疾病基因工程疫苗方向的研究，E-mail：zjp1986128@163.com,Tel:87283705;魏燕鸣（1973-），女，湖北汉川人，助理工程师，学士，主要从事预防兽医学相关工作，E-mail:weiyanming@sina.com,Tel:027-87282608,二人并列第一作者
基金资助:
国家863计划（2011AA10A210）;国家973计划 ( 2012CB518805)

Analysis of Immune Effect on Streptococcus suis-Haemophilus parasuis Bivalent Subunit Vaccine in Mice

ZHAO Jian-ping, WEI Yan-ming, SONG Dai-song, ZHANG Qiang, ZHU Jian, ZHOU Ming-guang, LI Ran, KANG Chao, JIN Mei-lin^*

（National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China）

Received:2012-02-16 Online:2012-10-25 Published:2012-10-25

摘要/Abstract

摘要： 本研究旨在开发一种用于防制猪链球菌病和副猪嗜血杆菌病的新型二联基因工程疫苗，并对其免疫效应进行评价。选择猪链球菌2型蛋白溶血素SLY、HP0197和副猪嗜血杆菌外膜蛋白D15和HPS06257 4种蛋白，重组表达并纯化，添加一定比例的佐剂制成含有不同组分的亚单位疫苗。研究表明该二联亚单位疫苗及各单苗能够引起小鼠良好的体液免疫，并能保护机体分别抵抗致死剂量猪链球菌2型（SS2)和副猪嗜血杆菌(HPS)强毒株的攻击。此外，调理吞噬试验表明疫苗高免血清能够有效杀死细菌，并赋予机体被动抵抗SS2和HPS感染的能力。研究证实制备的猪链球菌副猪嗜血杆菌二联亚单位疫苗在小鼠具备良好的免疫保护效力。

Abstract: This study aims to develop a new kind of bivalent genetic engineering vaccine used to control Streptococcus suis (SS) disease and Haemophilus parasuis (HPS) disease, and to evaluate the vaccine's immune effect. Four proteins, Streptococcus suis serotype 2 proteins SLY, HP0197, and Haemophilus parasuis outer membrane proteins D15 and HPS06257 are selected, recombinant expressed, purified and added adjuvant of certain proportion to be made with different components of subunit vaccines. In the present study, the Streptococcus suis serotype 2-Haemophilus parasuis (SS2-HPS) bivalent subunit vaccine and their single vaccines could elicit a significant humoral antibody response and confer significant protection against challenge with lethal dose of SS2 or HPS in mice, respectively. In addition, the hyperimmune sera against these vaccines could efficiently kill the bacteria in the opsonized phagocytosis test and confer significant protection against SS2 infection in the experiment of passive immunization. The study shows this SS-HPS bivalent subunit vaccine has good immune protection in mice.

中图分类号:

S852.61

赵建平，魏燕鸣，宋岱松，等. 猪链球菌-副猪嗜血杆菌二联亚单位疫苗在小鼠的免疫效力分析[J]. 畜牧兽医学报, doi: .

ZHAO Jian-ping, WEI Yan-ming, SONG Dai-song, et al. Analysis of Immune Effect on Streptococcus suis-Haemophilus parasuis Bivalent Subunit Vaccine in Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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