Abstract: The objective of the study was to detect the distribution of T329S mutation in 11 Chinese local chicken breeds. The FMO3 genotype frequency in 11 Chinese local chicken breeds including Beijing You, Hebei Chai, Gushi, Liyang, Rugao, Taihu, Huainan Mahuang, Silky, Dongxiang Blue-shelled, Wenchang and Tibetan chicken, were detected by PRC-RFLP method. The results showed that Dongxiang Blue-shelled chicken only had TT genotype, suggesting that no individual would lay fishy taint eggs in this breed. Fishy taint susceptible individuals with genotype SS were detected in all other 10 local breeds. Of these breeds, Beijing You chicken had the highest frequency of the SS genotype (8.8%) and Tibetan chicken present the lowest (0.7%). In conclusion, the frequencies of fishy taint genotype in Chinese local chicken breeds were generally low and the susceptible individuals could be easily detected by PCR-RFLP method