2005-2008年浙江省生猪主产区猪戊型肝炎血清流行病学调查

畜牧兽医学报 ›› 2009, Vol. 40 ›› Issue (7): 1037-1042.doi:

2005-2008年浙江省生猪主产区猪戊型肝炎血清流行病学调查

帅江冰¹，张晓峰¹，徐晶靓³，陈宁²，方维焕²*

1浙江出入境检验检疫局,杭州 310012；2浙江大学浙江省动物预防医学重点实验室，杭州 310029；3杭州市畜牧兽医局，杭州 310029

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-07-24 发布日期:2009-07-24
通讯作者: 方维焕

Seroprevalence of Hepatitis E Virus Infections in Pigs from Zhejiang Province During 2005-2008

SHUAI Jiangbing1, ZHANG Xiaofeng1, XU Jingjing3, CHEN Ning2, FANG Weihuan2*

1Zhejiang EntryExit Inspection and Quarantine Bureau, Hangzhou 310012，China;2Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029,China; 3Hangzhou Animal Husbandry and Veterinary Bureau, Hangzhou 310029,China

Received:1900-01-01 Revised:1900-01-01 Online:2009-07-24 Published:2009-07-24
Contact: FANG Weihuan

摘要/Abstract

摘要： 本研究旨在建立检测猪戊型肝炎病毒（HEV）感染的间接ELISA方法，并将其用于分析浙江省生猪主产区猪群的HEV感染状况。首先人工合成并原核表达了HEV ORF2蛋白的主要抗原表位区，免疫印迹显示表达产物对HEV抗体阳性的猪血清具有良好的反应性。以此纯化蛋白为包被抗原建立了猪血清中HEV特异性抗体的ELISA检测方法，检测结果显示其与商品化HEV抗体诊断试剂盒的检测符合率达915％。对2005-2008年采集自浙江省不同地区46个猪场的1 330份血清进行了检测，有741份血清检测为猪HEV抗体阳性，平均阳性率为557%。其中2005和2006年HEV抗体阳性率分别为627% (175/279)和614%（301/490），明显高于2007年的431%（59/137）和2008年的486%（206/424）。同时，66-100日龄中猪的血清HEV抗体阳性率（582%，110/189）显著高于30-65日龄小猪（397%，73/184）和101-160日龄大猪（441%，83/188）。从猪场水平来看，仅有3个猪场未检测到HEV抗体，场阳性率932%。结果表明本研究建立的ELISA方法切实可行，检测结果显示浙江省猪场中HEV感染相当普遍。

Abstract: The study was aimed to establish an indirect ELISA for epidemiological investigation of hepatitis E virus（HEV） infections in pigs from the major pig-producing regions of Zhejiang Province. The gene fragment covering immunogenic epitopes of HEV ORF2 was synthesized and expressed in Escherichia coli. Western blot analysis revealed that the purified protein reacted to HEV positive sera, but not to positive sera of other common viruses infecting pigs. An indirect ELISA system was then developed using truncated HEV ORF2 protein as the coating antigen and had diagnostic accuracy of 915 % as compared with a diagnostic kit for HEV antibodies. A total of 1 330 serum samples were collected from 46 pig farms in Zhejiang province during 2005-2008 and tested for seroprevalence of HEV using the indirect ELISA. The average HEVpositive rate was 557% (741/1 330). The positive rate of 2005 and 2006 was 627% (175/279) and 614% (301/490) respectively, much higher than those of 2007 (431%, 59/137) and 2008 (486%, 206/424). Meanwhile, the pigs aged 66-100 days had a statistically higher positive rate (582%, 110/189）as compared with that of pigs of 30-65 (397%, 73/184） and 101-160 (441%, 83/188) days. Only three herds in the study were HEV antibodynegative, indicating high seroprevalence of HEV in the pig populations in Zhejiang Province.

帅江冰;张晓峰;徐晶靓;陈宁;方维焕 . 2005-2008年浙江省生猪主产区猪戊型肝炎血清流行病学调查[J]. 畜牧兽医学报, 2009, 40(7): 1037-1042.

SHUAI Jiangbing;ZHANG Xiaofeng;XU Jingjing;CHEN Ning;FANG Weihuan. Seroprevalence of Hepatitis E Virus Infections in Pigs from Zhejiang Province During 2005-2008[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2009, 40(7): 1037-1042.

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