Abstract: Our study was conducted to isolate the candidate binding proteins of IBDV VP2. The VP2 cDNA was amplified by RTPCR and subcloned into the bait vector pSos in yeast twohybrid system. The plasmid DNAs from chicken bursal cDNA library were inserted into the prey vector. The recombinant bait and prey vectors were cotransformed into cdc25H for cDNA library screening using the yeast twohybrid system. After first round of screening, 48 putatively positive clones were obtained, among of which 19 clones were confirmed to be positive by the second round of screening. Among the 19 positive clones, 8 encoded polypeptides shared high homologies with known proteins, the Ras subfamily such as RRAS2, Rit1 and NRas, the TCTP and PARP14 are tumor proteins, Mx have antivirus activity, PRKX is a protein kinase, and LAMB3. The finding of candidated VP2binding proteins made foundation for research on IBDV receptor(s), apoptosis or the pathgenic merchanism of IBDV.