Abstract: To investigate the effect of inhibin gene immunization on follicle developments and reproductive hormone secretions,58 scalpers were immunized with 0 (Group C)，075 (T1), 150(T2), 225 (T3), and 300 mg(T4) of inhibin eukaryotic expression plasmid (pCIS) respectively. The results showed that animals in groups T1，T2, T3 and T4 had a greater number of large follicles (≥10 mm) than those in group C, and had similar number of medium follicles (7 mm≤d＜10 mm) or small (4 mm≤d＜7 mm) follicles(P>005) to those in the group C. Moreover，follicles on bilateral ovaries of scalpers in inhibin gene vaccine immunized groups except for group T1 (P>005)were significantly larger than those in group C （P<005）. The levels of follicle stimulating hormone (FSH) in peripheral blood sera of scalpers immunized were higher than those of the control animals, and the difference was significant after a booster immunization（P<005）. Similarly, the levels of estradiol (E2) and progesterone (P4) in peripheral blood sera of scalpers immunized increased after a booster immunization, which were higher than those in the control animals（P<005）. These results demonstrated that inhibin gene vaccine pCIS is able to stimulate FSH secretion, and to be beneficial to follicle development and maturation of scalpers.