Abstract: To investigate the effect of TSA on goat somatic cell nuclear transfer (SCNT)embryo development，we treated goat fetal fibroblasts for 24 h prior to nuclear transfer or reconstructed embryos for 10 h following oocyte activation with TSA.50 and 75 nmol·L-1 TSA treatment of donor cells increased blastocyst development compared to control （2734%，2689% vs 1618%，P<005）.Development to blastocysts by embryo cloned from 575 nmol·L-1 TSA treatment of reconstructed embryos was higher than that in embryos cloned from untreated control，moreover blastocyst rate of 50 nmol·L-1 group was significantly higher than that of control（2734% vs 1653%，P<005）.These results indicated that TSAtreatment can dramatically improve subsequent in vitro development of cloned embryos，and could be partially decrease the level of DNA methylation and histone deacetylation of donor cells.