Abstract: In this study, the cumulus cells were dealed with Trichostatin A(TSA), Roscovitine(ROS), serum starvation and contact inhibition, we detect the change of level of acetylation, the cell cycle, and the development of cloning embryos after the treatments, which provided academic foundation for raising efficiency of cloning. Using the above methods, the cell morphology change and the motility rate were observed, then the levels of acetylation and cell cycle were detected through the indirect immunofluorescence and the flow cytometry respectively, and the motility rate were more than 94% all. TSA obviously enhanced the level of acetylation (P<0.05); ROS also made a high level of acetylation, but lower than the TSA treatment (P<0.05), the serum starvation treatment was lower than the control. The cumulus cells after treatments had good morphology, TSA made the cell cycle stop at the G0/G1 and the highest cleavage and blastula rate (P<0.01, （85.2±3.4）% vs （68.6±6.7）%;（30.2±5.7）% vs （10.4±8.3）%). These results indicated that the cumulus cells treated with TSA not only had a high level of acetylation and better morphology change compared with the other groups, but also had the highest percent of blastula, so it was more suitable method to deal with the donor cells.