Abstract: Hexokinase (HK) Ⅱ is a key enzyme member in the glycolysis reaction which expressed mainly in muscle.In this study,the polymorphisms of MspⅠsite （A171G） in the 17th exon of HK2 gene in 186 pigs of six pig populations were analyzsed by using PCR-RFLP，and the relationship between the HK2 genotypes and pork quality, carcass traits was evaluated.The results showed that the frequencies of the two alleles (A and B) were similar in all studied homozygous and heterozygous pig breeds, and allele B was dominant. In addition, no AA genetype was found in six breeds, the statistically associations of AB and BB with carcass length, rib numbers, and lion eye area were very significant different (P<0.01), but the significant differences (P<0.05) of their genetype effects were found in backfat thickness at shoulder, backfat thickness at thorax-waist, and average backfat thickness over three positions, lion eye height, lion eye width, fat meat percentage, lean meat percentage and, intramuscular fat. It needs more and comprehensive studies to verify whether the polymorphic site could be used as the candidate site affecting carcass and meat quality traits in markers assisted selection.