Abstract: In order to study exon10 of prolactin receptor gene（PRLR）in alpacas, and to analyze the homology to other mammals, blood samples and tissue from 60 Huacaya alpacas, breed in China Alpacas Base, were taken and their genomic DNAs were extracted by the method of hydroxybenzene / chloroform, and then cloned, sequenced and submitted to GenBank（DQ206831）. The sequence analysis revealed that the alpaca prolactin receptor exon10 spaned 1 133 bp, and included 1 046 bp encoding region and 87 bp untranslated region. And the multiple sequence alignments revealed that the homology of alpacas is 80% to the other mammals ( such as cow, sheep, pig, dog, rabbit, rat and so on), the sequence of amnio acid is more than 66%, but it is only 40% to 45% to the fish at the amnio acid level.