畜牧兽医学报 ›› 2006, Vol. 37 ›› Issue (4): 317-320.doi:

• 遗传繁育 • 上一篇    下一篇

BoLA-DQADRB3* exon2多态性及其与奶牛乳房炎的关联分析

高树新;许尚忠;李金泉;高 雪;任红艳;陈金宝;马 云   

  1. 1.中国农业科学院畜牧研究所,北京 100094;2.内蒙古农业大学动物科学与技术学院,呼和浩特 010018;3.内蒙古民族大学动物科学与技术学院,通辽 028042; 4.西北农林科技大学动物科学与技术学院,杨凌 712100
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-04-25 发布日期:2006-04-25

Analysis of Polymorphisms of BoLA-DQA and DRB3* exon2 and the Relationship between the Gene and Mastitis in Dairy Cattle

GAO Shu-xin;XU Shang-zhong;LI Jin-quan;GAO Xue;REN Hong-yan;CHEN Jin-bao;MA Yun   

  1. 1. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094,China;2. College of Animal Science and Technology, Inner Mongolia Agricultural University, Huhhot 010018,China;3.College of Animal Science and Technology, Inner Mongolia University for Nationality, Tongliao 028042,China;4. College of Animal Science and Technology, Northwest Science ﹠ Technology University of Agriculture and Forestry, Yangling 712100, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-04-25 Published:2006-04-25

摘要: 采用PCR-SSCP技术,检测到9种中国荷斯坦牛BoLA-DQA*exon2基因基因型、15种BoLA-DRB3*exon2基因的基因型和3种单体型,分析比较了各基因型、单体型与奶牛乳房炎的关联性。结果在中国荷斯坦牛中,没有发现与乳房炎易感性或抗性有关联的基因和基因型,但发现DQA-B/DRB3-C、DQA-G/DRB3-F两种单体型可能与奶牛乳房炎易感性有关,而DQB-F/DRB3E单体型可能与奶牛乳房炎抗性有关。

Abstract: Nine genotypes of BoLA-DQA*exon2 allele, fifteen genotypes and three haplotypes of BoLA-DRB3* exon2 allele were observed in Chinese Holstein using PCR-SSCP. Correlations between all genotype or haplotype with the mastitis were analyzed in this study. The results showed that susceptible or resistant gene and genotype of mastitis was not observed in Chinese Holstein Cattle, DQA-B/DRB3-C and DQA-G/DRB3-F haplotypes might relate to the susceptibility of mastitis, DQB-F/DRB3-E haplotype may relate to the resistance of mastitis.