绵羊肺腺瘤病毒NM株前病毒gag基因的克隆与序列分析

畜牧兽医学报 ›› 2005, Vol. 36 ›› Issue (1): 54-57.doi:

绵羊肺腺瘤病毒NM株前病毒gag基因的克隆与序列分析

刘淑英; 马学恩; 李景鹏

1.内蒙古农业大学动物科学与医学学院, 呼和浩特 010018；2.东北农业大学生命中心基因部, 哈尔滨 150030

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-01-25 发布日期:2005-01-25

Cloning and Sequencing Analysis gag Gene of Jaagsiekte Sheep Retrovirus Inner Mongolia Strain

LIU Shu-ying; MA Xue-en; LI Jing-peng

1.College of Animal Science and Medicine,Inner Mongolia Agricultural University,Huhhot 010018; 2.College of Life Science,Northeast Agricultural University, Harbin 150030

Received:1900-01-01 Revised:1900-01-01 Online:2005-01-25 Published:2005-01-25

摘要/Abstract

摘要： 参照GenBank中已发表的绵羊肺腺瘤病毒（JSRV）的全基因序列，设计合成3对引物，对JSRV NM株的gag基因分3段进行PCR扩增，经琼脂糖凝胶电泳分析，分别呈3条531、888和949 bp的特异条带，将其分别克隆入pMD-18 T载体中，进行序列测定并拼接序列，得到完整的gag基因序列。分析结果表明，与南非代表株（基因序列号NC-001494）的gag基因序列比较，核苷酸同源性为89.0%，推导出的氨基酸同源性为90%。与美国代表株（基因序列号AF105220）的gag基因序列比较，核苷酸同源性为86.3%，氨基酸同源性为87%。

Abstract: In order to amplify gag gene of Jaagsiekte sheep retrovirus Inner Mongolia strain, three pairs of primers were designed according to the GenBank sequence . The fragments of gag gene was obtained by polymerase chain reaction(PCR),then the genes were cloned into pMD-18 T vector and identified by PstI, EcoRI and SalI digestion. The nucleotide and amino acid sequences of NM strain gag gene were compared with the counterpart sequences of South Africa strain(NC-001494) and USA strain (AF105220). The nucleotide and amino acid homology of gag gene were 89.0%,90% and 86.3%,87%, respectively.

刘淑英;马学恩; 李景鹏. 绵羊肺腺瘤病毒NM株前病毒gag基因的克隆与序列分析[J]. 畜牧兽医学报, 2005, 36(1): 54-57.

LIU Shu-ying; MA Xue-en; LI Jing-peng . Cloning and Sequencing Analysis gag Gene of Jaagsiekte Sheep Retrovirus Inner Mongolia Strain[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2005, 36(1): 54-57.

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