低浓度阿奇霉素对猪链球菌2型蛋白表达、荚膜多糖与药物敏感性的影响

doi:10.11843/j.issn.0366-6964.2023.02.032

畜牧兽医学报 ›› 2023, Vol. 54 ›› Issue (2): 757-765.doi: 10.11843/j.issn.0366-6964.2023.02.032

低浓度阿奇霉素对猪链球菌2型蛋白表达、荚膜多糖与药物敏感性的影响

杨艳北^1,2, 许晶^3*, 刘婉萍³, 陶艾妮³, 冯育林¹, 孙勇¹, 王闯⁴, 刘建⁴

1. 江西中医药大学博士后流动站/正邦集团有限公司博士后工作站, 南昌 330000;
2. 南昌师范学院生物技术研究院/江西省地方鸡种遗传改良重点实验室, 南昌 330000;
3. 南昌师范学院生命科学学院, 南昌 330000;
4. 江西正邦养殖有限公司, 南昌 330000

收稿日期:2022-05-30 出版日期:2023-02-23 发布日期:2023-02-21
通讯作者: 许晶,主要从事蛋白质组学和生物信息学研究,E-mail:1302522503@qq.com
作者简介:杨艳北(1986-),男,吉林长春人,讲师,博士,主要从事蛋白质组学和生物信息学研究,E-mail:731294789@qq.com;Tel:+86-791-87619332
基金资助:
江西省教育厅科学技术研究项目（GJJ202631）；南昌师范学院博士科研启动基金（NSBSJJ2018023）；南昌师范学院校级科研项目（22XJZR05）；南昌师范学院学生科研创新项目（21XSKY51）；江西省“双千人才”创新领军人才短期项目

Effects of Low-concentration Azitromycin on Protein Expression, Capsular Polysaccharides and Drug Susceptibility of Streptococcus suis Type 2

YANG Yanbei^1,2, XU Jing^3*, LIU Wanping³, TAO Aini³, FENG Yulin¹, SUN Yong¹, WANG Chuang⁴, LIU Jian⁴

1. Jiangxi University of Chinese Medicine Center for Post-Doctoral Studies/Zhengbang Group Co., Ltd., Post-Doctoral Research Center, Nanchang 330000, China;
2. Institute of Biotechnology, Nanchang Normal University/Jiangxi Province Key Laboratory of Genetic Improvement of Indigenous Chicken Breeds, Nanchang 330000, China;
3. School of Life Sciences, Nanchang Normal University, Nanchang 330000, China;
4. Jiangxi Zhengbang Breeding Co., Ltd., Nanchang 330000, China

Received:2022-05-30 Online:2023-02-23 Published:2023-02-21

摘要/Abstract

摘要： 本研究通过探索低浓度阿奇霉素对猪链球菌2型蛋白表达、荚膜多糖与药物敏感性的影响，为进一步研究环境中低浓度抗生素对微生物的影响奠定基础。用低浓度阿奇霉素处理猪链球菌2型，利用蛋白质组学iTRAQ技术，筛选关键差异表达蛋白。同时测定猪链球菌2型的荚膜多糖含量和药物敏感性。结果显示，共鉴定到差异表达蛋白174个，占总鉴定蛋白的11.6%，多数差异表达蛋白参与催化和代谢过程，属于膜蛋白，其中，3个荚膜多糖蛋白、9个ABC转运蛋白、1个50 S核糖体蛋白、1个核糖体RNA甲基转移酶、1个DNA回旋酶上调表达；8个荚膜多糖蛋白、4个50S核糖体蛋白、4个30S核糖体蛋白、1个核糖体RNA甲基转移酶、1个DNA聚合酶IV下调表达。用低浓度阿奇霉素处理猪链球菌2型，发现其对多种抗生素的敏感性降低，存活下来的菌株，恢复正常培养后，药物敏感性恢复。用低浓度阿奇霉素处理猪链球菌2型，荚膜多糖含量也未发生大幅度变化。猪链球菌2型通过改变自身蛋白质组的表达量，以适应低浓度阿奇霉素的选择压力。与低浓度阿奇霉素共存时，猪链球菌2型开启外排泵系统，会增加细菌产生多重耐药的风险。

关键词: 猪链球菌2型, 阿奇霉素, 荚膜多糖, 药物敏感性

Abstract: This study explored the effects of low-concentration azithromycin on protein expression, capsular polysaccharide and drug susceptibility of Streptococcus suis type 2, so as to lay a foundation for further study on the effects of low-concentration antibiotics in the environment on microorganisms. After S. suis type 2 was exposed to low-concentration azithromycin, the key differentially expressed proteins were screened by proteomic iTRAQ technology. At the same time, the capsular polysaccharide content and drug susceptibility of S. suis type 2 were determined. One hundred and seventy-four differentially expressed proteins were identified, accounting for 11.6% of the total identified proteins. Most differentially expressed proteins were involved in catalytic and metabolic processes. Most differentially expressed proteins belonged to membrane proteins. Among them, three capsular polysaccharide proteins, nine ABC transporters, one 50S ribosomal protein, one ribosomal RNA methyltransferase and one DNA gyrase were up-regulated; eight capsular polysaccharide proteins, four 50S ribosomal proteins, four 30S ribosomal proteins, one ribosomal RNA methyltransferase and one DNA polymerase IV were down regulated. When S. suis type 2 was exposed to low-concentration azithromycin, S. suis type 2 had reduced sensitivity to a variety of antibiotics. The drug sensitivity of the surviving strains recovered after returning to normal culture. When S. suis type 2 was exposed to low-concentration azithromycin, the content of capsular polysaccharide did not change significantly. S. suis type 2 adapted to the selection pressure of azithromycin by changing the expression of its own proteome. When coexisting with low-concentration azithromycin, S. suis type 2 started the efflux pump system. The efflux effect would increase the risk of bacteria producing multiple drug resistance.

Key words: Streptococcus suis type 2, azithromycin, capsular polysaccharide, drug susceptibility

中图分类号:

杨艳北, 许晶, 刘婉萍, 陶艾妮, 冯育林, 孙勇, 王闯, 刘建. 低浓度阿奇霉素对猪链球菌2型蛋白表达、荚膜多糖与药物敏感性的影响[J]. 畜牧兽医学报, 2023, 54(2): 757-765.

YANG Yanbei, XU Jing, LIU Wanping, TAO Aini, FENG Yulin, SUN Yong, WANG Chuang, LIU Jian. Effects of Low-concentration Azitromycin on Protein Expression, Capsular Polysaccharides and Drug Susceptibility of Streptococcus suis Type 2[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 757-765.

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低浓度阿奇霉素对猪链球菌2型蛋白表达、荚膜多糖与药物敏感性的影响

Effects of Low-concentration Azitromycin on Protein Expression, Capsular Polysaccharides and Drug Susceptibility of Streptococcus suis Type 2

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