关中奶山羊源D型产气荚膜梭菌全基因组序列测定及其分子特征分析

doi:10.11843/j.issn.0366-6964.2022.11.022

畜牧兽医学报 ›› 2022, Vol. 53 ›› Issue (11): 3967-3974.doi: 10.11843/j.issn.0366-6964.2022.11.022

关中奶山羊源D型产气荚膜梭菌全基因组序列测定及其分子特征分析

吴克, 冯航, 王娟*, 杨增岐*

西北农林科技大学动物医学院, 杨凌 712100

收稿日期:2022-04-25 出版日期:2022-11-23 发布日期:2022-11-25
通讯作者: 杨增岐,主要从事预防兽医学研究,E-mail:yzq1106@nwsuaf.edu.cn;王娟,主要从事预防兽医学研究,E-mail:juan.wang1234@hotmail.com
作者简介:吴克(1997-),男,河北邯郸人,硕士生,主要从事预防兽医学研究,E-mail:1351332788@qq.com
基金资助:
财政部和农业农村部：国家现代农业产业技术体系资助（CARS-39）

Whole Genome Sequencing and Molecular Characterization Analysis of Clostridium perfringens Type D Strains from Guanzhong Dairy Goats

WU Ke, FENG Hang, WANG Juan*, YANG Zengqi*

College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China

Received:2022-04-25 Online:2022-11-23 Published:2022-11-25

摘要/Abstract

摘要： 本研究自陕西省富平县某规模化关中奶山羊养殖场腹泻奶山羊肛门拭子中分离到5株产气荚膜梭菌，命名为21-D-1~21-D-5。毒素基因检测表明，其均为携带etx的D型产气荚膜梭菌，且21-D-5携带食源性致病毒素基因cpe。全基因组序列测定显示，5株D型产气荚膜梭菌基因组大小、GC含量和基因数量稳定；单核苷酸多态性（SNPs）分析显示，21-D-1和21-D-2以及21-D-3和21-D-4之间的SNPs差异极低（<25），表明其大概率属于相同的产气荚膜梭菌菌株。分离的D型产气荚膜梭菌基因组中共检测到15种毒素基因，其中，毒素基因etx在分离的D型菌株中高度保守、基因环境相似，且在菌株21-D-5中毒素基因cpe位于etx下游。此外，包括噁唑烷酮类耐药基因optrA在内的9种耐药基因也在分离株中被检测到，并且erm（A）、optrA和fexA具有共同传播的可能性。本研究为国内首次对D型产气荚膜梭菌进行全基因组序列测定分析，结果对产气荚膜梭菌疾病的防治和基因组的进一步研究具有参考价值。

关键词: 关中奶山羊, D型产气荚膜梭菌, 全基因组序列测定分析

Abstract: In our study, five Clostridium perfringens isolates were obtained from diarrhea Guanzhong diary goats and named as 21-D-1 to 21-D-5. Toxin genes detection indicated the isolates all belonged to C. perfringens type D positive for etx and cpa, while cpe associated with foodborne diseases was detected in strain 21-D-5. After whole genome sequencing, we found the genome size, GC content and genes number were stable among the C. perfringens type D genomes. SNPs analysis showed there were few SNPs (<25) between 21-D-1 and 21-D-2, and 21-D-3 and 21-D-4, which revealed they might belonged to two C. perfringens strains, respectively. Fifteen toxin gens were detected among the C. perfringens type D isolates, sequence and genetic environment analysis of etx indicated sequence of etx were highly conserved and the genetic environments were similar among the genomes. In addition, cpe was detected downstream etx in the genome of C. perfringens strain 21-D-5. Besides, oxazolidinones-resistant gene optrA and the other eight antimicrobial resistant genes were also detected among the isolates, and erm(A), optrA and fexA were observed to have the the possibility of co-spread. This is the first study in China, which whole genome sequence and analyze the genomes of C. perfringens type D strains. The results of our study may provide reference for prevention and treatment of C. perfringens diseases, and further researches on C. perfringens genomes.

Key words: Guanzhong dairy goats, Clostridium perfringens type D, whole genome sequencing

中图分类号:

S852.616.3

吴克, 冯航, 王娟, 杨增岐. 关中奶山羊源D型产气荚膜梭菌全基因组序列测定及其分子特征分析[J]. 畜牧兽医学报, 2022, 53(11): 3967-3974.

WU Ke, FENG Hang, WANG Juan, YANG Zengqi. Whole Genome Sequencing and Molecular Characterization Analysis of Clostridium perfringens Type D Strains from Guanzhong Dairy Goats[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 3967-3974.

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关中奶山羊源D型产气荚膜梭菌全基因组序列测定及其分子特征分析

Whole Genome Sequencing and Molecular Characterization Analysis of Clostridium perfringens Type D Strains from Guanzhong Dairy Goats

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