牛传染性鼻气管炎病毒诱导MDBK细胞线粒体损伤模型的建立

doi:10.11843/j.issn.0366-6964.2022.09.027

畜牧兽医学报 ›› 2022, Vol. 53 ›› Issue (9): 3132-3139.doi: 10.11843/j.issn.0366-6964.2022.09.027

牛传染性鼻气管炎病毒诱导MDBK细胞线粒体损伤模型的建立

郭雪萍¹, 马英才¹, 李泽龙¹, 王添姿¹, 高函雅¹, 徐李依娜¹, 吴煜锋¹, 钟旗³, 姚刚^1*, 马雪连^1,2*

1. 新疆农业大学动物医学学院, 乌鲁木齐 830052;
2. 新疆农业大学畜牧学博士后流动站, 乌鲁木齐 830052;
3. 新疆畜牧科学院兽医研究所, 乌鲁木齐 830000

收稿日期:2021-12-20 出版日期:2022-09-23 发布日期:2022-09-23
通讯作者: 姚刚,主要从事动物生长发育与畜产品质量安全,E-mail:yg@xjau.edu.cn;马雪连,主要从事动物保健与畜产品质量安全研究,E-mail:13699381790@163.com
作者简介:郭雪萍(1998-),女,新疆沙雅人,硕士生,主要从事动物保健与畜产品质量安全研究,E-mail:g15199210269@163.com
基金资助:
国家自然科学基金（3200190591）；自治区天山青年基金（2020Q075）；肉牛高效健康饲养技术集成研究与示范（2021A02003-1）

Establishment of a Model of Mitochondrial Damage Induced by Bovine Infectious Rhinotracheitis Virus in MDBK Cells

GUO Xueping¹, MA Yingcai¹, LI Zelong¹, WANG Tianzi¹, GAO Hanya¹, XU-Li Yina¹, WU Yufeng¹, ZHONG Qi³, YAO Gang^1*, MA Xuelian^1,2*

1. College of Animal Medicine, Xinjiang Agricultural University, Urumqi 830052, China;
2. The Post Doctoral Station of Animal Husbandry Science, Xinjiang Agricultural University, Urumqi 830052, China;
3. Institute of Veterinary Medicine, Xinjiang Academy of Animal Sciences, Urumqi 830000, China

Received:2021-12-20 Online:2022-09-23 Published:2022-09-23

摘要/Abstract

摘要： 为探讨牛传染性鼻气管炎病毒（IBRV）诱导MDBK细胞线粒体损伤的最适时间和浓度，本试验用0.5、1.0、1.5、2.0感染复数（multiplicity of infection，MOI） IBRV感染MDBK细胞6、12、24、36、48 h，采用透射电镜、流式细胞术等技术检测MMP和MPTP异常开放情况。结果显示，用不同MOI IBRV感染MDBK细胞12 h，感染组与对照组相比，MPTP均显著降低，且除2.0 MOI外均随接毒剂量的增加而降低；MMP在1.5 MOI后开始极显著降低；用1.5 MOI IBRV分别感染MDBK细胞6、12、24、36 h，感染组与对照组相比MPTP及MMP从6 h起均显著降低，且随着病毒感染时间的不断延长而降低。透射电镜结果显示，IBRV感染MDBK细胞6 h就可引起细胞线粒体损伤，出现部分空泡化，24 h线粒体完全空泡化，并且在线粒体周围可以看见病毒粒子。结果表明，IBRV感染可以诱导MDBK细胞线粒体损伤，且用1.5 MOI感染MDBK细胞6～24 h是诱导细胞线粒体损伤的最佳时间和浓度。该时间和浓度可用于IBRV相关药物研究，为进一步研究线粒体损伤提供理论依据。

关键词: IBRV, 线粒体损伤, MMP, MPTP

Abstract: The purpose of this study was to investigate the optimal time and concentration of bovine infectious rhinotracheitis virus (IBRV)-induced mitochondrial damage in MDBK cells. 0.5, 1.0, 1.5 and 2.0 MOI IBRV were used to infect MDBK cells for 6, 12, 24, 36 and 48 h. Electron microscopy and flow cytometry were used to detect the MMP and abnormal opening of MPTP. The results showed that when MDBK cells were infected with different MOI IBRV for 12 h, the MPTP decreased significantly in the Infection group compared with the control group, and decreased with the increase of exposure dose except 2.0 MOI;The MMP started to decrease significantly after 1.5 MOI; When the MDBK cells were infected with 1.5 MOI IBRV for 6, 12, 24 and 36 h respectively, compared with the control group, the MPTP and MMP in the Infection group were significantly decreased from 6 h, and decreased with the prolongation of the virus infection;The results of transmission electron microscopy showed that MDBK cells infected with IBRV could cause mitochondrial damage and partial vacuolization after 6 h, complete vacuolization of mitochondria after 24 h, and virus particles could be seen around mitochondria. The results show that IBRV infection can induce mitochondrial damage in MDBK cells, and infecting MDBK cells at 1.5 MOI for 6-24 h is the best time and concentration to induce cell mitochondrial damage. This time and concentration can be used for IBRV-related drug research, and provide a theoretical basis for further research on mitochondrial damage.

Key words: IBRV, mitochondrial damage, MMP, MPTP

中图分类号:

S852.659.1

郭雪萍, 马英才, 李泽龙, 王添姿, 高函雅, 徐李依娜, 吴煜锋, 钟旗, 姚刚, 马雪连. 牛传染性鼻气管炎病毒诱导MDBK细胞线粒体损伤模型的建立[J]. 畜牧兽医学报, 2022, 53(9): 3132-3139.

GUO Xueping, MA Yingcai, LI Zelong, WANG Tianzi, GAO Hanya, XU-Li Yina, WU Yufeng, ZHONG Qi, YAO Gang, MA Xuelian. Establishment of a Model of Mitochondrial Damage Induced by Bovine Infectious Rhinotracheitis Virus in MDBK Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3132-3139.

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牛传染性鼻气管炎病毒诱导MDBK细胞线粒体损伤模型的建立

Establishment of a Model of Mitochondrial Damage Induced by Bovine Infectious Rhinotracheitis Virus in MDBK Cells

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