线粒体tRNA-Lys(T7719G)基因变异对绵羊产羔数的影响及遗传学分析

doi:10.11843/j.issn.0366-6964.2022.06.010

摘要/Abstract

摘要： 旨在揭示线粒体tRNA-Lys(T7719G)基因变异对小尾寒羊为母本的杂交羊群产羔数的影响，探讨将该基因变异作为分子标记辅助选种的可行性。本研究以小尾寒羊为母本的健康适龄繁殖母羊为研究对象，利用PCR扩增产物测序判定基因型，开展tRNA-Lys(T7719G)基因多态性及季节、胎次对产羔数影响的遗传学分析。结果表明，线粒体tRNA-Lys基因有G、T两种等位基因，等位基因频率分别为57.8%、42.2%;携带G等位基因的母羊较携带T等位基因的母羊平均胎产羔数多0.08只。在相同季节，群体产羔数随胎次以及G基因型母羊个体数量增加而增加。建立了胎产羔数预测模型方程：胎产羔数=1.201+(－0.009)×虚拟变量1+0.043×虚拟变量2+0.194×虚拟变量3+0.061×胎次+0.123×携带G等位基因母羊数量，即羊群中增加一个携带G等位基因的个体，胎产羔数预期增加0.123只。线粒体tRNA-Lys(T7719G)基因可以作为小尾寒羊为母本的杂交羊群产羔数性状的分子标记。

关键词: 线粒体, tRNA-Lys基因, 绵羊, 产羔数, 遗传学分析

Abstract: The aim of the present study was to investigate the effect of mitochondrial tRNA-Lys(T7719G) gene variation on the litter size in sheep population with Small-tailed Han sheep as female parent, and to explore the possibility of mitochondrial tRNA-Lys(T7719G) gene variation as molecular marker for assisted selection. Healthy and suitable breeding ewe with Small tail-Han sheep as female parent were selected. The polymorphism analysis of tRNA-Lys(T7719G) gene was conducted by sequencing of PCR amplificatin product, genetic analysis of the influence of tRNA-Lys (T7719G) gene, season and parity on the litter size were conducted. The results showed that the mitochondrial tRNA-Lys gene mutated into G and T alleles, with allele frequency of 57.8% and 42.2%, respectively. The average litter size of ewes with G allele was 0.08 more than that of ewes with T allele in average litter size. Litter size of population increased with the number of parities and the number of individual ewes with G allele in the same season. The formula of prediction model of litter size was established: Litter size = 1.201 + (－0.009)×dummy variable 1 + 0.043×dummy variable 2 + 0.194×dummy variable 3 + 0.061×parity + 0.123×ewes number carrying G allele, which indicated that adding an individual with G allele in population, the litter size was expected to increase 0.123. tRNA-Lys(T7719G) gene can be used as a molecular marker for litter size traits of population with Small-tailed Han sheep as female parent.

Key words: mitochondrion, tRNA-Lys gene, sheep, litter size, genetic analysis

中图分类号:

S826.2

吕世琪, 马晓菲, 李兵, 贾春晖, 王艳超, 田树军, 周荣艳, 陈晓勇. 线粒体tRNA-Lys(T7719G)基因变异对绵羊产羔数的影响及遗传学分析[J]. 畜牧兽医学报, 2022, 53(6): 1759-1767.

Lü Shiqi, MA Xiaofei, LI Bing, JIA Chunhui, WANG Yanchao, TIAN Shujun, ZHOU Rongyan, CHEN Xiaoyong. Genetics Analysis and Effects of Mitochondrial tRNA-Lys (T7719G) Variation on Litter Size of Sheep[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1759-1767.

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