绵羊mtDNA变异与产羔数关联分析

doi:10.11843/j.issn.0366-6964.2021.07.007

摘要/Abstract

摘要： 旨在分析线粒体DNA变异与产羔数性状的关系，寻找影响绵羊繁殖性能的分子遗传标记，为高繁殖力绵羊的选育提供理论依据和方法。本研究以有产羔记录的健康小尾寒羊多羔母羊（multiple Small tailed han sheep，XM）、湖羊多羔母羊（multiple Hu sheep，HM）、蒙古羊双羔母羊（twins Mongolian sheep，MT）、蒙古羊单羔母羊（singletons Mongolian sheep，MS）、欧拉羊双羔母羊（twins Oula sheep，OT）、欧拉羊单羔母羊（singletons Oula sheep，OS）、甘肃高山细毛羊双羔母羊（twins Gansu alpine fine wool sheep，GT）和甘肃高山细毛羊单羔母羊（singletons Gansu alpine fine wool sheep，GS）为研究对象，通过PCR和测序法检测试验羊mtDNA多态性，并结合产羔数进行关联性分析。结果表明，在A1099T、T1112C、C13576T、T13837C和T13855C位点，XM与HM之间基因型分布基本一致，XM与MS、OT、OS、GT、GS之间基因型分布均有显著性差异（P<0.05）。在T15668C位点，MT与MS之间基因型分布有显著性差异（P<0.05）。在T15445C、T15627C、T15657C、T15732C、G15977A、A16101G和C16429T位点，OS与OT之间基因型分布均有显著性差异（P<0.05）。在A15923G位点，GT与GS之间基因型分布有显著性差异（P<0.05）。T15668C变异位点显著影响蒙古羊产羔数（P<0.05）；T15445C、T15627C、T15657C、T15732C、T15956C、G15977A和C16429T变异位点显著影响欧拉羊产羔数（P<0.05）；A15923G位点显著影响甘肃高山细毛羊产羔数（P<0.05）。单倍型Hap_2（TTCTTACGC）和Hap_4（TTTTTATGC）对试验绵羊产羔数也具有一定的影响。

关键词: 绵羊, 线粒体DNA, 产羔数

Abstract: The aim of this study was to analyze the relationship between mitochondrial DNA variation and litter size, and to find the molecular genetic markers affecting the reproductive perfor-mance of sheep, so as to provide a theoretical basis and method for the selection and breeding of sheep with high reproductive performance. Multiple Small tailed han sheep (XM), multiple Hu sheep (HM), twins Mongolian sheep (MT), singletons Mongolian sheep (MS), twins Oula sheep (OT), singletons Oula sheep (OS), twins Gansu alpine fine wool sheep (GT) and singletons Gansu alpine fine wool sheep (GS) all with a record of lambing were used as the research subjects. The mtDNA polymorphism was detected by PCR and sequencing, and the correlation analysis was conducted in combination with the litter size. The results showed that the genotype distribution between XM and HM was basically the similar at A1099T, T1112C, C13576T, T13837C and T13855C sites, and the genotype distribution between XM and MS, OT, OS, GT, GS were significantly different (P<0.05). There was significant difference in genotype distribution between MT and MS at T15668C site (P<0.05). There were significant differences in genotype distribution between OS and OT at T15445C, T15627C, T15657C, T15732C, G15977A, A16101G and C16429T sites (P<0.05). And there was significant difference in genotype distribution between GT and GS at A15923G site(P<0.05). The T15668C variable site significantly affected the litter size of Mongolian sheep (P<0.05). The variable sites of T15445C, T15627C, T15657C, T15732C, T15956C, G15977A and C16429T significantly affected the litter size of Oula sheep (P<0.05). Also, the A15923G site significantly affected the litter size of Gansu alpine fine wool sheep (P<0.05). What's more, Hap_2 (TTCTTACGC) and Hap_4 (TTTTTATGC) haplotypes also had certain effects on the litter size.

Key words: sheep, mitochondrial DNA, litter size

中图分类号:

S826.2

肖帆, 张利平, 吴建平, 靳继鹏, 孙渭博, 张筱艳. 绵羊mtDNA变异与产羔数关联分析[J]. 畜牧兽医学报, 2021, 52(7): 1831-1844.

XIAO Fan, ZHANG Liping, WU Jianping, JIN Jipeng, SUN Weibo, ZHANG Xiaoyan. Analysis of Correlation between Mitochondrial DNA Variation and Litter Size in Sheep[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(7): 1831-1844.

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