制动应激对孕猪海马结构神经元可塑性的影响

doi:10.11843/j.issn.0366-6964.2021.012.026

摘要/Abstract

摘要： 旨在研究限位栏饲养造成的制动应激对妊娠母猪神经元可塑性的影响，本研究选取6头8~9月龄配种成功的巴马小型母猪，随机分成两组，应激组（n=3）限位栏饲养，对照组（n=3）自由环境饲养，其余饲养管理一致。于妊娠第18天处死孕猪，取其海马和血液，使用ELISA、放射免疫法检测相关激素水平，制作海马石蜡切片进行尼氏染色和镀银染色后观察神经元丢失率、树突复杂度以及树突棘数量等，并采用Western blot检测海马脑源性神经营养因子（BDNF）表达水平。结果显示，应激组血浆中促肾上腺皮质激素释放激素（CRH）、促肾上腺皮质激素（ACTH）、皮质醇（COR）水平均显著上升（P≤0.05），齿状回（DG）和CA3区出现神经元丢失现象，CA3区锥形细胞树突复杂度下降，神经元成熟树突棘和未成熟树突棘数量均显著降低，DG的成熟树突棘数量显著降低，且海马内的BDNF蛋白表达量较对照组降低70.6%（P≤0.01）。因此，制动应激减弱了孕猪大脑海马中CA3区和DG的神经元可塑性。

关键词: 制动应激, 孕猪, 海马, 神经元可塑性

Abstract: In order to study the effect of restraint stress on the neuroplasticity of pregnant sows, 6 Bama miniature sows aged 8-9 months were randomly divided into two groups:stress group (n=3) and control group (n=3). The remaining conditions were consistent.The pregnant pigs were sacrificed on the 18th day of gestation. The hippocampal and blood samples were collected and the hormone levels were detected by ELISA and radioimmunoassay.After the paraffin-embedded hippocampal sections were prepared and stained by Nishner staining and silver staining, the neuron loss rate, dendritic complexity and the number of dendritic spines were observed, and the expression level of brain-derived neurotrophic factor (BDNF) in the hippocampus was detected by Western blot. The results showed that the plasma levels of corticotropin-releasing hormone (CRH), adrenocorticotrophic (ACTH) and cortisol (COR) increased significantly in the stress group (P ≤ 0.05), in the hippocampus dentate gyrus (DG) and CA3 area neurons leakage phenomenon, CA3 area down cone cells dendritic complexity, mature dendritic spines of neurons and the quantity of immature dendritic spines are significantly reduced, DG mature dendritic spines significantly reduced, and the amount of BDNF protein expression in hippocampus was reduced by 70.6% (P ≤ 0.01). Therefore, restraint stress attenuates the neuronal plasticity of CA3 and DG in the hippocampus of pregnant pigs.

Key words: immobilization stress, pregnant pig, the hippocampus, neuronal plasticity

中图分类号:

Q421

陈思彦, 孙维婧, 王子旭, 陈耀星, 曹静, 董玉兰. 制动应激对孕猪海马结构神经元可塑性的影响[J]. 畜牧兽医学报, 2021, 52(12): 3598-3608.

CHEN Siyan, SUN Weijing, WANG Zixu, CHEN Yaoxing, CAO Jing, DONG Yulan. Effect of Restraint Stress on Hippocampal Neuron Plasticity in Pregnant Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(12): 3598-3608.

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