2017—2019年华东地区猪场主要病毒性腹泻病原调查

doi:10.11843/j.issn.0366-6964.2020.12.023

畜牧兽医学报 ›› 2020, Vol. 51 ›› Issue (12): 3141-3150.doi: 10.11843/j.issn.0366-6964.2020.12.023

2017—2019年华东地区猪场主要病毒性腹泻病原调查

常新见^1,2, 周金柱¹, 殷杰^1,3, 牛贝贝^1,4, 范宝超¹, 郭容利¹, 赵永祥¹, 牛家强², 何孔旺¹, 李彬^1*

1. 江苏省农业科学院兽医研究所, 南京 210014;
2. 西藏农牧学院动物科学学院, 林芝 860000;
3. 内蒙古民族大学动物科学技术学院, 通辽 028000;
4. 南京农业大学动物医学院, 南京 210014

收稿日期:2020-03-16 出版日期:2020-12-25 发布日期:2020-12-23
通讯作者: 李彬,主要从事猪病毒性腹泻防控技术研究,E-mail:libinana@126.com
作者简介:常新见(1992-),男,河南商丘人,硕士生,主要从事猪预防兽医方向研究,E-mail:2899327994@qq.com;周金柱(1986-),男,山西朔州人,助理研究员,主要从事动物腹泻防控技术,E-mail:syzhoujz@163.com。
基金资助:
国家重点研发计划（2018YFD0500101）；国家自然科学基金（31872481）；江苏省人兽共患病学重点实验室资助项目（R1907）

Investigation on Pathogens of Major Viral Diarrhea in Pig Farms in East China from 2017 to 2019

CHANG Xinjian^1,2, ZHOU Jinzhu¹, YIN Jie^1,3, NIU Beibei^1,4, FAN Baochao¹, GUO Rongli¹, ZHAO Yongxiang¹, NIU Jiaqiang², HE Kongwang¹, LI Bin^1*

1. Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
2. College of Animal Science, Tibet Agriculture and Animal Husbandry College, Linzhi 860000, China;
3. College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao 028000, China;
4. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210014, China

Received:2020-03-16 Online:2020-12-25 Published:2020-12-23

摘要/Abstract

摘要： 本研究旨在了解当前我国华东地区引起猪腹泻的主要病毒性腹泻病原的流行情况，为该地区更好地开展猪腹泻病毒的防控工作提供临床数据。分别采用RT-PCR检测方法对2017—2019年华东地区35个场别594份临床样品检测猪流行性腹泻病毒（porcine epidemic diarrhea virus，PEDV）、猪轮状病毒（porcine rotavirus，PoRV）、猪传染性胃肠炎病毒（transmissible gastroenteritis virus of swine，TGEV）、猪丁型冠状病毒（porcine deltacoronavirus，PDCoV）和猪萨佩罗病毒（porcine Sapelovirus，PSV）等5种猪的病毒性腹泻病原，并对其阳性率及混合感染情况进行统计分析。结果显示，2017—2019年的PEDV、PDCoV、PoRV、TGEV及PSV的平均阳性率为分别为62.6%（372/594）、27.9%（166/594）、16.8%（100/594）、13.3%（79/594）及3.87%（23/594），二重混合感染中以PEDV+PDCoV及PEDV+PoRV为主，平均混合感染率分别为22.4%（133/594）和13.3%（79/594）。三重感染中以PEDV+PoRV+PDCoV混合感染为主，感染率为7.58%（45/594），未见四重及五重病原混合感染。2017—2019年PEDV平均阳性检出率最高，且常与PoRV及PDCoV发生混合感染，是当前华东地区猪病毒性腹泻类疫病的防控重点。TGEV和PDCoV在健康育肥猪及母猪的检出率中占比较大，表明隐性感染变得普遍，值得养殖户关注。除此之外，PSV的检出率远低于其他4种腹泻病毒。总之，随着2018年后养殖场加强生物安全防控措施和猪群管理，各病原检出率和阳性场检出率呈现逐年下降趋势。

关键词: 猪病毒性腹泻, PEDV, 混合感染, 华东地区

Abstract: To investigate the epidemic situation of viral diarrhea in East China in recent years, and provide epidemiological survey data for comprehensive prevention, 549 fecal samples were collected in 2017-2019 from diarrhea pigs in 5 cities of East China and RT-PCR were used to detect porcine epidemic diarrhea virus (PEDV), porcine rotavirus (PoRV), porcine transmissible gastroenteritis virus (TGEV), porcine deltacoronavirus (PDCoV) and porcine Sapelovirus (PSV), and statistical analysis of its positive rate and mixed infection was performed.The results showed that during 2017 and 2019, PEDV,PDCoV,PoRV,TGEV and PSV positive rate were 62.6% (372/594),27.9% (166/594),16.8% (100/594),13.3% (79/594), and 3.87% (23/594), respectively.In the double mixed infection, the average mixed infection rate of PEDV + PDCoV and PEDV + PoRV were the main ones, which were 22.4% (133/594) and 13.3% (79/594), respectively. Among the triple infections, the mixed infection rate of PEDV + PoRV + PDCoV was dominated by 7.58% (45/594), and there were no mixed infections of four and five pathogens. The highest positive detection rate of PEDV was during 2017 and 2019, and mixed infection with PoRV and PDCoV, which was currently the focus of prevention and control of viral diarrhea in East China. TGEV and PDCoV accounted for a large proportion of the detection rate of healthy fattening pigs and sows, indicating that recessive infections had become common and deserve attention from farmers. In addition, the detection rate of PSV was more lower than the other four diarrhea viruses. On the whole, since August 2018 that the farms strengthened biosecurity prevention and control measures, the detection rate of various pathogens and the detection rate of sites have shown a downward trend year by year.

Key words: porcine viral diarrhea, PEDV, mixed infection, East China

中图分类号:

常新见, 周金柱, 殷杰, 牛贝贝, 范宝超, 郭容利, 赵永祥, 牛家强, 何孔旺, 李彬. 2017—2019年华东地区猪场主要病毒性腹泻病原调查[J]. 畜牧兽医学报, 2020, 51(12): 3141-3150.

CHANG Xinjian, ZHOU Jinzhu, YIN Jie, NIU Beibei, FAN Baochao, GUO Rongli, ZHAO Yongxiang, NIU Jiaqiang, HE Kongwang, LI Bin. Investigation on Pathogens of Major Viral Diarrhea in Pig Farms in East China from 2017 to 2019[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(12): 3141-3150.

参考文献 38

[1]	王颢然,高利,高翔,等.2008-2018年我国猪流行性腹泻病毒混合感染分析[J].中国动物传染病学报,2020,28(3):97-103.WANG H R,GAO L,GAO X,et al.Analysis of mixed infections of porcine epidemic diarrhea virus with other pathogens from 2008 to 2018 in China[J].Chinese Journal of Animal Infectious Diseases,2020,28(3):97-103.(in Chinese)
[2]	杨莉,杨茂生,杨昌艳,等.贵州猪腹泻的流行病学调查[J].上海畜牧兽医通讯,2015(4):58.YANG L,YANG M S,YANG C Y,et al.Epidemiological investigation of pigs diarrhea in Guizhou[J].Shanghai Journal of Animal Husbandry and Veterinary Medicine,2015(4):58.(in Chinese)
[3]	SONG D,PARK B.Porcine epidemic diarrhoea virus:a comprehensive review of molecular epidemiology,diagnosis,and vaccines[J].Virus Genes,2012,44(2):167-175.
[4]	LI Z L,ZHU L,MA J Y,et al.Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field strains in South China[J].Virus Genes,2012,45(1):181-185.
[5]	董波,张寄香,戴爱玲,等.闽西地区仔猪病毒性腹泻流行情况调查[J].江苏农业科学,2018,46(12):144-146.DONG B,ZHANG J X,DAI A L,et al.Investigation on the prevalence of piglet viral diarrhea in western Fujian[J].Jiangsu Agricultural Sciences,2018,46(12):144-146.(in Chinese)
[6]	刘吉山,王玉茂,林初文,等.猪病毒性腹泻流行现状及防控对策[J].黑龙江畜牧兽医,2012(22):81-83.LIU J S,WANG Y M,LIN C W,et al.Epidemiological status of swine viral diarrhea and prevention and control measures[J].Heilongjiang Animal Science and Veterinary Medicine,2012(22):81-83.(in Chinese)
[7]	ZHAI S L,WEI W K,LI X P,et al.Occurrence and sequence analysis of porcine deltacoronaviruses in southern China[J].Virol J,2016,13(1):136.
[8]	ZHANG X,SHI H Y,CHEN J F,et al.Identification of cellular proteome using two-dimensional difference gel electrophoresis in ST cells infected with transmissible gastroenteritis coronavirus[J].Proteome Sci,2013,11(1):31.
[9]	DING L,HUANG Y,DU Q,et al.TGEV nucleocapsid protein induces cell cycle arrest and apoptosis through activation of p53 signaling[J].Biochem Biophys Res Commun,2014,445(2):497-503.
[10]	PENSAERT M B,DE BOUCK P.A new coronavirus-like particle associated with diarrhea in swine[J].Arch Virol,1978,58(3):243-247.
[11]	DEBOUCK P,PENSAERT M.Experimental infection of pigs with a new porcine enteric coronavirus,CV777[J].Am J Vet Res,1980,41(2):219-223.
[12]	PAN Y F,TIAN X Y,LI W,et al.Isolation and characterization of a variant porcine epidemic diarrhea virus in China[J].Virol J,2012,9(1):195.
[13]	佟有恩,冯力,李伟杰,等.猪传染性胃肠炎与猪流行性腹泻二联弱毒疫苗的研究[J].中国预防兽医学报,1999,21(6):406-410.TONG Y E,FENG L,LI W J,et al.Development of bi-combined attenuated vaccine against transmissible gastroenteritis virus and porcine epidemic diarrhea virus[J].Chinese Journal of Preventive Veterinary Medicine,1999,21(6):406-410.(in Chinese)
[14]	LI G W,CHEN Q,HARMON K M,et al.Full-length genome sequence of porcine deltacoronavirus strain USA/IA/2014/8734[J].Genome Announc,2014,2(2):e00278-14.
[15]	CHEN F Z,ZHU Y X,WU M Z,et al.Full-length genome characterization of Chinese porcine deltacoronavirus strain CH/SXD1/2015[J].Genome Announc,2015,3(5):e01284-15.
[16]	MA Y M,ZHANG Y,LIANG X Y,et al.Origin,evolution,and virulence of porcine deltacoronaviruses in the United States[J].mBio,2015,6(2):e00064-15.
[17]	吴秋玉.新现猪Delta冠状病毒RT-PCR检测方法的建立及其应用[J].农业开发与装备,2017(7):78,142.WU Q Y.Establishment and application of RT-PCR detection method for new porcine Delta coronavirus[J].Agricultural Development and Equipments,2017(7):78,142.(in Chinese)
[18]	LEE S,LEE C.Complete genome characterization of Korean porcine deltacoronavirus strain KOR/KNU14-04/2014[J].Genome Announc,2014,2(6):e01191-14.
[19]	WOODE G N,BRIDGER J C,HALL G,et al.The isolation of a reovirus-like agent associated with diarrhoea in colostrum-deprived calves in Great Britain[J].Res Vet Sci,1974,16(1):102-105.
[20]	刘洪云,黄均健,严仲烈,等.猪轮状病毒组织培养的研究[J].上海农业科技,1981(3):31-32,4.LIU H Y,HUANG J J,YAN Z L,et al.Study on tissue culture of porcine rotavirus[J].Shanghai Agricultural Science and Technology,1981(3):31-32,4.(in Chinese)
[21]	BOHL E H.Rotaviral diarrhea in pigs:brief review[J].J Am Vet Med Assoc,1979,174(6):613-615.
[22]	PARASHAR U D,HUMMELMAN E G,BRESEE J S,et al.Global illness and deaths caused by rotavirus disease in children[J].Emerg Infect Dis,2003,9(5):565-572.
[23]	LAMONT P H,BETTS A O.Enteroviruses in the pig[J].Nature,1958,182(4635):608-609.
[24]	DONIN D G,DE ARRUDA LEME R,ALFIERI A F,et al.First report of Porcine teschovirus (PTV),Porcine sapelovirus (PSV) and Enterovirus G (EV-G) in pig herds of Brazil[J].Trop Anim Health Prod,2014,46(3):523-528.
[25]	SON K Y,KIM D S,KWON J,et al.Full-length genomic analysis of Korean porcine sapelovirus strains[J].PLoS One,2014,9(9):e107860.
[26]	LAN D L,JI W H,YANG S X,et al.Isolation and characterization of the first Chinese porcine sapelovirus strain[J].Arch Virol,2011,156(9):1567-1574.
[27]	焦点,孙杰,俞正玉,等.江苏及周边地区猪流行性腹泻病毒的检测及其ORF3基因序列分析[J].畜牧与兽医,2018,50(10):88-94.JIAO D,SUN J,YU Z Y,et al.Detection of PEDV in Jiangsu and its surrounding areas and sequence analysis of the viral ORF3 gene[J].Animal Husbandry and Veterinary Medicine,2018,50(10):88-94.(in Chinese)
[28]	逄凤娇,俞正玉,何孔旺,等.猪Deltacoronavirus RT-PCR检测方法的建立及其应用[J].中国预防兽医学报,2015,37(9):683-686.PANG F J,YU Z Y,HE K W,et al.Development and application of a RT-PCR assay for detection of porcine Deltacoronavirus[J].Chinese Journal of Preventive Veterinary Medicine,2015,37(9):683-686.(in Chinese)
[29]	孙杰,刘畅,朱琳,等.猪萨佩罗病毒RT-PCR检测方法的建立与应用[J].畜牧与兽医,2018,50(7):118-121.SUN J,LIU C,ZHU L,et al.Development and application of an RT-PCR assay for detection of porcine sapelovirus[J].Animal Husbandry and Veterinary Medicine,2018,50(7):118-121.(in Chinese)
[30]	朱子健,闫丽辉,鞠妍,等.2015~2016年我国东北地区猪病毒性腹泻流行病学调查[J].中国预防兽医学报,2017,39(5):356-360.ZHU Z J,YAN L H,JU Y,et al.The epidemiological investigation on the viral diarrhea in pigs in the Northeast region of China during 2015-2016[J].Chinese Journal of Preventive Veterinary Medicine,2017,39(5):356-360.(in Chinese)
[31]	宋亚兵,徐帅飞,苏丹萍,等.2012年~2016年广东省猪丁型冠状病毒的流行病学调查及分析[J].中国预防兽医学报,2018,40(10):886-890.SONG Y B,XU S F,SU D P,et al.Epidemiological survey and analysis of porcine deltacoronavirus in Guangdong Province from 2012 to 2016[J].Chinese Journal of Preventive Veterinary Medicine,2018,40(10):886-890.(in Chinese)
[32]	徐丽华,李军星,苏菲,等.2011-2017年浙江省猪病毒性腹泻病的流行病学调查[J].中国兽医科学,2018,48(5):625-630.XU L H,LI J X,SU F,et al.Epidemiological investigation on the viral diarrhea in pigs in Zhejiang Province during 2011-2017[J].Chinese Veterinary Science,2018,48(5):625-630.(in Chinese)
[33]	王淞,曾昊,陈智,等.山东省猪腹泻病例中PEDV、TGEV和PRV的感染调查与分析[J].中国畜牧兽医,2017,44(7):2165-2170.(in Chinese) WANG S,ZENG H,CHEN Z,et al.Investigation and analysis of porcine epidemic diarrhea virus,transmissible gastroenteritis virus and pseudorabies virus of swine diarrhea cases in Shandong Province[J].China Animal Husbandry and Veterinary Medicine,2017,44(7):2165-2170.(in Chinese)
[34]	仇国明.猪病毒性腹泻病流行病学调查[J].今日畜牧兽医,2019,35(9):59,3.QIU G M.An epidemiological survey of porcine viral diarrhea[J].Today Animal Husbandry and Veterinary Medicine,2019,35(9):59,3.(in Chinese)
[35]	黄宇.非洲猪瘟传播途径的分析与防控[J].广西畜牧兽医,2019,35(3):115-118.HUANG Y.Analysis and prevention of the spread of African swine fever[J].Guangxi Journal of Animal Husbandry & Veterinary Medicine,2019,35(3):115-118.(in Chinese)
[36]	张椿.猪流行性腹泻的诊断与预防[J].今日畜牧兽医,2019,35(2):32.ZHANG C.Diagnosis and prevention of swine epidemic diarrhea[J].Today Animal Husbandry and Veterinary Medicine,2019,35(2):32.(in Chinese)
[37]	岩纳.猪病毒性腹泻的流行特点及防控措施[J].中国动物保健,2019,21(2):53-54.YAN N.The epidemic characteristics and prevention and control measures of swine viral diarrhea[J].China Animal Health,2019,21(2):53-54.(in Chinese)
[38]	张传亮,陈星宇,赵灵燕,等.浙江省猪病毒性腹泻流行病学调查及防控对策[J].中国动物检疫,2013,30(7):47-48.ZHANG C L,CHEN X Y,ZHAO L Y,et al.Epidemiological investigcation of and control strategies for swine viral diarrhea[J].Chinese Journal of Animal Health Inspection,2013,30(7):47-48.(in Chinese)

2017—2019年华东地区猪场主要病毒性腹泻病原调查

Investigation on Pathogens of Major Viral Diarrhea in Pig Farms in East China from 2017 to 2019

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