Gα12蛋白抑制口蹄疫病毒在PK-15细胞上复制

doi:10.11843/j.issn.0366-6964.2020.03.015

摘要/Abstract

摘要： 鸟嘌呤核苷酸结合蛋白Gα12参与多种细胞反应的调节，但是在口蹄疫病毒（foot-and-mouth disease virus，FMDV）方面的研究较少。本研究中，用FMDV感染PK-15细胞，发现Gα12的mRNA和蛋白水平都有所上调，预示Gα12可能与FMDV复制存在某种联系，需要进一步研究Gα12蛋白对FMDV在PK-15细胞上复制的影响。为此，在过表达Gα12和干扰Gα12表达的情况下，通过实时荧光定量、Western blot和TCID₅₀检测Gα12对FMDV复制的影响。结果表明，过表达Gα12后，FMDV的mRNA水平、蛋白水平和病毒滴度都有所降低，然而，下调Gα12后，FMDV的复制呈相反的趋势，说明Gα12可以抑制FMDV在PK-15细胞上的复制。本研究首次发现Gα12具有抑制口蹄疫病毒复制的作用，为抗FMDV的研究提供了新思路和理论基础，对FMDV相关研究具有重要意义。

关键词: Gα12, 口蹄疫病毒, 复制

Abstract: The guanine nucleotide binding protein Gα12 is involved in the regulation of various cellular responses, but there are few studies on foot-and-mouth disease virus (FMDV). In this study, we had used FMDV to infect PK-15 cells, and found that the mRNA and protein levels of Gα12 were up-regulated, indicating that Gα12 may have some relationships with FMDV replication. To investigate the effect of Gα12 on the replication of FMDV, quantitative real-time PCR, Western blot and TCID₅₀ were used to detect the effect of overexpression of Gα12 and knockdown of Gα12 on FMDV replication. The results showed that the mRNA level, protein level and virus titer of FMDV were decreased after overexpression of Gα12. However, when knockdown Gα12, the replication of FMDV showed the opposite effect, indicating that Gα12 inhibited the replication of FMDV. This study found for the first time that Gα12 has the effect of inhibiting the replication of FMDV, and provides a new idea and theoretical basis for the study of anti-FMDV, which is of great significance for FMVD-related research.

Key words: Gα12, foot-and-mouth disease virus, replication

中图分类号:

S852.659.6

王志芳, 张伟, 杨帆, 魏婷, 郑海学. Gα12蛋白抑制口蹄疫病毒在PK-15细胞上复制[J]. 畜牧兽医学报, 2020, 51(3): 556-564.

WANG Zhifang, ZHANG Wei, YANG Fan, WEI Ting, ZHENG Haixue. Gα12 Suppresses the Replication of Foot-and-mouth Disease Virus in PK-15 Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(3): 556-564.

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