多不饱和脂肪酸日粮中添加酵母硒对湖羊脂肪酸组成和抗氧化的影响

doi:10.11843/j.issn.0366-6964.2018.08.011

摘要/Abstract

摘要：

旨在研究富含多不饱和脂肪酸（PUFAs，添加紫苏籽）添加酵母硒对湖羊肌肉和肝脂肪酸组成及抗氧化的影响。本研究选取3月龄、体重为（23.02±1.36）kg的湖羊公羔45只，随机分为3组，分别为基础日粮组（C）、C+10%紫苏籽组（PFS）和C+PFS+酵母硒组（PFS+Se）。预试期10 d，正试期60 d。试验开始和结束时测定羔羊生产性能，颈静脉采血进行生化分析，同时在试验结束时进行屠宰试验，取背最长肌和肝，测定脂肪酸组成、抗氧化酶活性和抗氧化基因表达量。结果表明：1）多不饱和脂肪酸日粮中添加酵母硒对湖羊生长性能和屠宰性能均无显著性影响（P>0.05）。2）日粮中添加紫苏籽和酵母硒均显著提高湖羊背最长肌粗脂肪含量（P<0.05），但对Fe、Cu、Na、K和Mg等矿物质元素含量无显著影响（P>0.05）。3）日粮中添加紫苏籽和酵母硒均显著增加肌肉和肝n-3多不饱和脂肪酸含量，降低n-6/n-3的比值（P<0.05）。4）在紫苏籽日粮中添加酵母硒显著提高了血清CAT活性和T-AOC（P<0.05），降低了肝中丙二醛含量（MDA，P<0.05），提高了肝中CAT和GPX基因的表达量（P<0.05）。上述结果表明，在紫苏籽日粮中添加酵母硒可提高湖羊机体的抗氧化能力，但不能促进PUFA沉积。因此，酵母硒可能不是促进饲喂紫苏籽日粮湖羊PUFA沉积最有效的方式，需进一步探究有效的抗氧化剂。

Abstract:

The aim of this study was to examine the effect of selenium yeast in containing polyunsaturated fatty acids dietary(supplemented perilla frutescens seed) on fatty acid composition and antioxidant capacity in muscle and liver of Hu lambs. Forty-five lambs of 3 months old with the body weight of (23.02±1.36) kg were selected and randomly divided into 3 groups:basic ration group(C), C+10% perilla frutescens seed group (PFS) and C+PFS+yeast selenium group(PFS+Se). The duration of preliminary experiment was 10 days and the duration of the formal experiment was 60 days. Growth performance was measured at the beginning and end of the trial and blood samples were collected for biochemical analysis. Slaughter test was performed at the end of the trail to collect longissimus dorsi muscle and liver samples for measuring the fatty acid composition, antioxidant enzyme activity and antioxidant related genes expression levels. The results showed that:1) There was no significant difference in growth performance and slaughter performance among different treatments (P>0.05). 2) The crude fat content in longissimus dorsi muscle of Hu sheep in the PFS and PFS+Se groups significantly increased (P<0.05), but there was no significantly difference in the content of mineral elements such as Fe, Cu, Na, K and Mg among different treatments (P>0.05). 3) The content of n-3 polyunsaturated fatty acid in liver and longissimus dorsi muscle in PFS group were significantly increased (P<0.05), and the values of n-6/n-3 in muscle and liver was significantly lower compared with the C group (P<0.05). 4) The CAT activity and T-AOC in the serum in PFS+Se group were significantly increased (P<0.05), the content of MDA in liver was significantly decreased (P<0.05), and the expressions of CAT and GPX in liver were significantly increased compared with C group (P<0.05). The above results show that adding selenium yeast in PUFAs dietary can improve the antioxidant capacity of Hu sheep, but it can not promote the PUFA deposition. Therefore, selenium yeast may not be the most effective way to promote the PUFA deposition in Hu sheep fed with perilla frutescens seed diet, and further work is needed to explore the effective antioxidants.

中图分类号:

S826.5

樊懿萱, 邓凯平, 澹台文静, 黄欣爱, 王锋, 陈明. 多不饱和脂肪酸日粮中添加酵母硒对湖羊脂肪酸组成和抗氧化的影响[J]. 畜牧兽医学报, 2018, 49(8): 1661-1673.

FAN Yi-xuan, DENG Kai-ping, TANTAI Wen-jing, HUANG Xin-ai, WANG Feng, CHEN Ming. Effect of Selenium Yeast in Containing PUFAs Dietary on Fatty Acid Composition and Antioxidant Capacity of Hu Lambs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1661-1673.

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