畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (12): 1919-1925.doi: 10.11843/j.issn.0366-6964.2013.12.009

• 遗传繁育 • 上一篇    下一篇

维生素E对无血清培养种母鸡颗粒细胞凋亡的影响

张宏馨1,邸科前1,陈辉2,黄仁录2*   

  1. (1.河北大学医学实验中心,保定 071000; 2.河北农业大学动物科技学院,保定 071000)
  • 收稿日期:2013-07-12 出版日期:2013-12-23 发布日期:2013-12-23
  • 通讯作者: 黄仁录,教授,博士生导师,E-mail: dkhrl@hebau.edu.cn
  • 作者简介:张宏馨(1971-),女,河北衡水人,博士,副教授,主要从事动物遗传育种与繁殖的营养调控研究,E-mail: lzwen5653@163.com
  • 基金资助:

    国家蛋鸡产业技术体系(CARS-41-K18)

Protective Effect of Vitamin E against Cell Apoptosis Induced by Serum-free Culturing in Granulosa Cells of Breeding Hen

ZHANG Hong-xin1, DI Ke-qian1,CHEN Hui2, HUANG Ren-lu2*   

  1. (1.Medical Experiment Center of Hebei University, Baoding 071000, China;2.College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071000, China)
  • Received:2013-07-12 Online:2013-12-23 Published:2013-12-23

摘要:

本试验旨在研究维生素E对无血清培养诱导的种母鸡卵泡颗粒细胞凋亡的影响。试验选用健康、有规则产蛋周期的高峰期种母鸡,收集小黄卵泡,分离颗粒细胞。将颗粒细胞经含0.5%FBS预培养后,用维生素E浓度为10 mg·L-1的无血清培养液继续培养。用流式细胞仪检测颗粒细胞凋亡和细胞周期分布情况。结果表明,与对照组相比,10 mg·L-1维生素E可以降低颗粒细胞的凋亡率(P0.01),使处于S期的细胞比例增加(P0.05)。结果提示,维生素E可以促进种母鸡颗粒细胞由G0/G1期进入S期,使更多颗粒细胞通过G1/S期限制点,从而抑制无血清培养所诱导的种母鸡颗粒细胞凋亡。

Abstract:

This study was conducted to investigate the effect of vitamin E (VE) against the cell apoptosis caused by serum-free culturing in granulosa cells from hen follicle. The granulosa cells were collected from the small yellow follicles of health breeding hens, which were at the egg producing peak within the regular cycle. After pre-incubation in 0.5% FCS mediumthe cells were cultured in serum-free medium containing 10 mg·L-1 vitamin E. The cell apoptosis and the cell cycle distribution of granulosa cells were detected by Flow Cytometry. The results showed that the concentration 10 mg·L-1 of vitamin E reduced the apoptosis of granulosa cells (P0.01), and increased the percentage of S phase (P0.05) compared with the control group. The results suggest that vitamin E can promote granulosa cells from G0/G1 phase to S phase and promote granular cells passing through G1/S period limit. Thereby the granulosa cell apoptosis of breeding hens induced by serum-free culture was inhibited.

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