ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2005, Vol. 36 ›› Issue (12): 1347-1350.

• 研究简报 • Previous Articles     Next Articles

The Examination of Camel β-defensin-1 (caBD-1) Gene’s Expression in Tissues

YANG Yin-feng;YU Xing-bang;TANG Bo; CAO Gui-fang; FAN Guang-li   

  1. 1.Laboratory of Animal Embryo and Developmental Biology, InnerMongolia Agricultural University, Huhhot 010018，China;2.Hulunbeier Veterinary Station, Hailaer 012008，China；3. Collge of Animal Science and Technology, Northwest Science and Technology University of Agriculture and Forestry, Yangling 712100，China
  • Received:1900-01-01 Revised:1900-01-01 Online:2005-12-25 Published:2005-12-25

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Abstract: Mammalian β-defensins are endogenous cysteine-rich peptide antibiotics that are produced either by epithelial cells lining the respiratory, digestive and urogenital tracts or by granulocytes and macrophages. Here, we examined the expressive tissues of caBD-1 mRNA in camels through RNA in situ hybridization using the digoxigenin-labeled antisense nucleic acid probe which is 42 bp and was designed and synthesized according the known caBD-1 cDNA sequence.The result indicates that caBD-1 mRNA express in the stratified squamous epithelium of dorsal tongue and the columnar epithelium of intestinal crypts.

Key words: β-defensin, camel, in situ hybridization