ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2008, Vol. 39 ›› Issue (1): 12-15.

• 遗传繁育 • Previous Articles     Next Articles

Cloning and PCR-RFLP Analysis of the Dopamine Receptor D4 Gene Partial Sequence of Horses

FAN Cai-yun;MANG Lai;CHENG Jian-bo;MORI Yu-ji   

  1. 1.College of Animal Science, Henan Institute of Science and Technology, Xinxiang 453003,China；2.College of Animal Science and Veterinary , Inner Mongolia Agricultural University , Huhhot 010018 , China; 3.College of Animal Science and Technology , China Agricultural University , Beijing 10094 , China; 4. Laboratory of Veterinary Ethology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-01-24 Published:2008-01-24

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Abstract: In this paper, partial sequence of horse DRD4 gene was cloned including intron1, partial exon1 and exon2. And restriction enzyme Stu I was used to analyze the polymorphism of the DRD4 gene sequences of 270 horses from 6 types including importing breed, cultivating breed and local breed. The products of digestion with restriction enzyme StuⅠ were detected by 8% nondenatured polyacrylamide gel electrophoresis and showed in silver staining protocol.The result indicated restriction enzyme Stu I showed polymorphism. Six kinds of genotypes were found in six populations , which were controlled by three alleles. The results of χ2 test showed that genotypes frequency of horse DRD4 gene in TB, SH, XN did not fit with Hardy-Weinberg equilibrium(P < 0.05), but that in WS, BH and WZ fit with Hardy-Weinberg equilibrium(P＞0.05) .

Key words: horses, DRD4 gene, cloning, polymorphism, PCR-RFLP