ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2008, Vol. 39 ›› Issue (8): 1040-1044.

• 遗传繁育 • Previous Articles     Next Articles

FSH Regulating the Expression of GDNF of Piglet Testis Sertoli Cellby Activing ERK1/2

SUN Yan1,2,WANG Xian-zhong1,2, WU Jian-yun1, BAI Ru-lan1, ZHANG Jia-hua1,2   

  1. 1.Chongqing Key Laboratory of Forage & Herbivore, Southwest University,Chongqing 400716,China；2.College of Animal Science and Technology,Southwest University,Chongqing 400716，China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-08-24 Published:2008-08-24
  • Contact: ZHANG Jia-hua

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Abstract: Primary piglet sertoli cell (SC) were used to study the effects of FSH on regulating Glial cell linederived neurotrophic factor (GDNF) expression. The results showed that: (1) The FSH-stimulated GDNF expression in the cultured SC was time- and concentration-dependent: the level of GDNF protein reached its highest when FSH (50 ng/mL) stimulated 1h. (2) FSH（50 ng/mL）or dbcAMP（100 μmol/L）could active ERK1/2 prominently along with a maximum stimulation at 2 h; (3) U0126 (MEK1/2 inhibitor) could apparently inhibited the activing effect of FSH on GDNF. These results suggested that, by activing the cAMPPKAERK1/2 and promting the transcription of GDNF gene, FSH stimulated the expression of GDNF of sertoli cells.

Key words: FSH, sertoli cell（SC）, GDNF protein, ERK1/2