ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2008, Vol. 39 ›› Issue (1): 108-112.

• 研究简报 • Previous Articles     Next Articles

Stduy on Characteristics of Sodium-dependent Phosphate Intake in Small Intestine of Growing Pig

SUN Jie;ZHANG Tie-ying;CHEN Zhi-wei;HAN Jin-cheng;LIU Zhi-wei   

  1. 1College of Life Science, Shandong University of Technology, Zibo 255049,China; 2State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094,China; 3College of Animal Science and Technology, Northwestern A & F University , Yangling 712100,China; 4College of Animal Science and Technology, Hebei Agricultural University, Baoding, 071001,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-01-24 Published:2008-01-24

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Abstract: Six 3-month-old growing Yorkshire pigs were killed to obtain mucosa, which used to prepare epithelium brush-border membrane vesicles (BBMV). The dependence of Pi uptake on pH and sodium concentration were discussed and kinetics of Na+-dependent Pi uptake into BBMV of growing pig were estimated by the measurement of phosphate uptake into BBMV within 15 s at room temperature. Results showed that acid pH made a negative effect on both sodium-dependent phosphate uptake and passive diffusion. Phosphate uptake into BBMV was strongly dependent on sodium concentration. Hill coefficient (napp) of sodium-dependent phosphate cotransport system was 2.07, which indicated that at least two sodium ion was transported with each phosphate ion. The differences of Pi uptake into BBMV between different intestinal segments were not significant. On the condition of varying Pi concentration (0-2.0 mmol/L) and pH7.4, the Pi concentration that yielded halfmaximal transport (apparent Km) estimated was (0.43±0.09)mmol/L in Yorkshire BBMV. The maximal phosphate uptake into BBMV was (2.25±0.19)nmol/(mg protein·15 s).

Key words: growing pig, BBMV, Pi uptake