KDM2B在小鼠卵母细胞及早期胚胎发育过程中的表达分析

doi:10.11843/j.issn.0366-6964.2019.01.011

Abstract

Abstract:

The aim of this study was to investigate the expression pattern of KDM2B in mouse oocytes and preimplantation embryos, which provided important foundation for the mechanism study of KDM2B in meiosis and embryonic development. Twenty mice aged 6-8 weeks were selected as experimental material, GV and MⅡoocytes, 2-cell, 4-cell, 8-cell, blastocyst were collected. The primer was designed based on Mus musculus KDM2B sequence in GenBank, and the expression pattern of KDM2B was detected by RT-qPCR in different stages of preimplantation embryos. Then immunofluorescence staining was used to locate the distribution of KDM2B protein in embryos of each period. The result showed that the mRNA expression level of KDM2B in GV oocyte was extremely significant higher than that in MⅡ(P<0.01). During the early embryonic development, the KDM2B was expressed in 2-cell to blastocyst, and extremely significant higher in blastocyst than that in other stages (P<0.01). In preimplantation embryos, KDM2B was observed in the nucleolar at GV oocyte stage, and fluorescence signatures in the nucleus clearly weakened at the MⅡ stage (P<0.01), however KDM2B was not detected in 2-to 8-cell, and became detectable at blastocyst. This study established the spatial and temporal expression profiles of KDM2B, and the specific mechanism of KDM2B in regulating meiosis and embryonic development requires further investigation.

Key words: KDM2B, oocyte, preimplantation embryo, expression profile

CLC Number:

S814.8

YANG Xianying, XIONG Xianrong, HAN Jie, HUANG Xiangyue, WANG Yan, A GUO Yueda, LI Jian. Expression Analysis of KDM2B in Mouse Oocytes and Preimplantation Embryos[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 86-93.

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Expression Analysis of KDM2B in Mouse Oocytes and Preimplantation Embryos

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